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Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.)
- Source :
- Ultrasonics sonochemistry. 51
- Publication Year :
- 2018
-
Abstract
- The present study was conducted to evaluate the influence of ultrasound on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). For this purpose, optimized conditions (temperature 50 °C, time 150 min, solid to liquid ratio 1 g:15 ml, 70% amplitude and 240 W, 20 kHz frequency, 3 s on and 3 s off) of ultrasound (US) and conventional extraction (AGE: Agitation, water bath for 150 min, 50 °C at 150 rmp) were used. The results showed significant (p 0.05) effect of US and AGE over TFlaCs and PACs were observed. Moreover, the results of Fourier-transform infrared spectroscopy (FTIR) showed little changes in spectrum and US does not affect the functional group of bioactive compounds structure. Additionally, extracts (500–2000 µg/ml) protect pBR322 plasmid DNA damage induced by (1 mM H2O2 and 1 mM FeSO4), plasma oxidation (induced by 250 µM CuCl2) and inhibit erythrocyte hemolysis (induced by 200 mM AAPH, 34.6 to 66.73%). Sonication can be applied successfully for the extraction of bioactive compounds from plant materials with high biological activities.
- Subjects :
- Liquid ratio
Xanthine Oxidase
Erythrocytes
Acoustics and Ultrasonics
Sonication
Infrared spectroscopy
02 engineering and technology
Chemical Fractionation
010402 general chemistry
01 natural sciences
Hemolysis
Antioxidants
Inorganic Chemistry
Phenols
Chemical Engineering (miscellaneous)
Environmental Chemistry
Animals
Radiology, Nuclear Medicine and imaging
Proanthocyanidins
Fourier transform infrared spectroscopy
Chromatography
biology
business.industry
Chemistry
Plant Extracts
Clitoria ternatea
Organic Chemistry
Extraction (chemistry)
Ultrasound
Temperature
Erythrocyte hemolysis
021001 nanoscience & nanotechnology
biology.organism_classification
0104 chemical sciences
Ultrasonic Waves
0210 nano-technology
business
Clitoria
Oxidation-Reduction
Subjects
Details
- ISSN :
- 18732828
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Ultrasonics sonochemistry
- Accession number :
- edsair.doi.dedup.....0af07ee1d9c06665e90eba63c17fb26a