Back to Search
Start Over
Antibiotic susceptibility and mechanisms of beta-lactam resistance among clinical strains of Pseudomonas aeruginosa: first report in Algeria
- Source :
- Médecine et Maladies Infectieuses, Médecine et Maladies Infectieuses, Elsevier Masson, 2008, 38 (4), pp.187-91. ⟨10.1016/j.medmal.2008.01.009⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- National audience; OBJECTIVE: Pseudomonas aeruginosa is a major causative agent of hospital infections. Studies on this subject being rare in Algeria, we determined the antibiotic susceptibility of P. aeruginosa and investigated the mechanisms of beta-lactam resistance and the spread of multidrug resistant strains in the university affiliated Hospital of Tlemcen (Algeria). DESIGN: One hundred and ninety-nine consecutive strains of P. aeruginosa were collected between November 2005 and February 2007. MICs of antibiotics were measured by the agar dilution method. The resistance mechanisms to beta-lactams were identified phenotypically or by molecular methods (isoelectrofocusing, PCR and sequencing). Strains expressing a secondary beta-lactamase were serotyped and genotyped (Random Amplified Polymorphic DNA). RESULTS: The proportion of susceptible isolates were: ticarcillin (56%), piperacillin-tazobactam (81%), ceftazidime (88%), cefepime (80%), aztreonam (64%), imipenem (65%), amikacin (83%), tobramycin (81%) and ciprofloxacin (97%) according to the French CASFM breakpoints. Resistance to beta-lactams was linked to the production of transferable beta-lactamases (16%), overproduction of cephalosporinase AmpC (12%) and/or non-enzymatic mechanisms such as the loss of porin OprD (35%) and overproduction of the active efflux system MexAB-OprM (24%). High level resistance to ticarcillin was due to the expression of beta- lactamase OXA-10 alone or associated with TEM-110. A genotypic analysis revealed the spread of a multidrug resistant epidemic clone expressing these two acquired beta-lactamases in the surgical ICU. CONCLUSIONS: This study shows that resistance to antibiotics, in particular to imipenem of P. aeruginosa, is becoming a cause of concern in the Hospital of Tlemcen.
- Subjects :
- Imipenem
Genotype
Lactams
Hospital Departments
Ceftazidime
Drug resistance
Aztreonam
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
Hospitals, University
MESH: Genotype
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Drug Resistance, Bacterial
MESH: Drug Resistance, Bacterial
medicine
polycyclic compounds
030212 general & internal medicine
Serotyping
MESH: Hospitals, University
0303 health sciences
MESH: Microbial Sensitivity Tests
MESH: Lactams
030306 microbiology
Pseudomonas aeruginosa
MESH: Serotyping
biochemical phenomena, metabolism, and nutrition
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Multiple drug resistance
Infectious Diseases
chemistry
Amikacin
Ticarcillin
Algeria
MESH: Pseudomonas aeruginosa
MESH: Hospital Departments
MESH: Algeria
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 0399077X
- Database :
- OpenAIRE
- Journal :
- Médecine et Maladies Infectieuses, Médecine et Maladies Infectieuses, Elsevier Masson, 2008, 38 (4), pp.187-91. ⟨10.1016/j.medmal.2008.01.009⟩
- Accession number :
- edsair.doi.dedup.....0aeeba9661fb5807693430271d8256a2