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Application of Bone Morphogenetic Protein 7 Enhanced the Osteogenic Differentiation and Mineralization of Bone Marrow-Derived Stem Cells Cultured on Deproteinized Bovine Bone
- Source :
- Coatings, Vol 11, Iss 642, p 642 (2021), Coatings, Volume 11, Issue 6
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The growth of bone morphogenetic protein 7 (BMP-7) has been applied for tissue regeneration due to its osteoinductive properties. The aim of this research is to analyze the enhancing effects of BMP-7 on the osteogenic differentiation and mineralization of human bone marrow-derived stem cells cultured on the bovine bone particle. After the stem cells were loaded onto the bone graft material, their morphology was observed on day 7. Viability assays based on the application of fluorescent stains were used for qualitative analyses. Alkaline phosphatase activity assays and Alizarin red staining were used for the assessment of osteogenic differentiation on days 7 and 14. Next-generation mRNA sequencing was applied to evaluate global gene expression. Gene ontology and pathway analysis was used to propose the underlying mechanism. Fibroblast-like morphology was attained with the stem cells. The cells were shown to be firmly attached to the bone particle. Most of the stem cells produced an intense green fluorescence. The relative cellular viability assay values for BMP-7 groups at 0, 10, and 100 ng/mL on day 7 were 0.295 ± 0.003, 0.250 ± 0.002, and 0.240 ± 0.003, respectively (p &lt<br />0.05). Alkaline phosphatase activity was significantly higher in BMP-7 groups at concentration of 100 ng/mL compared to the control on days 7 and 14 (p &lt<br />0.05). The results of the mineralization assay showed significantly higher values for BMP-7 groups at 100 ng/mL concentration when compared with the control (p &lt<br />0.05). The expression of RUNX2 was increased with application of BMP-7 and mitogen-activated protein kinase pathway was associated with the target genes. Overall, this study shows that in vitro application of BMP-7 increases alkaline phosphorylase activity and mineralization of stem cells culture on deproteinized bovine bone mineral. The study suggests that combining stem cells with osteoinductive growth factors with scaffolds can have synergy effects on osteogenic differentiation.
- Subjects :
- Materials science
bone marrow
Cellular differentiation
bone morphogenetic protein 7
Surfaces and Interfaces
Engineering (General). Civil engineering (General)
Molecular biology
cell survival
Surfaces, Coatings and Films
osteogenesis
RUNX2
Bone morphogenetic protein 7
cell differentiation
MRNA Sequencing
medicine.anatomical_structure
stem cells
Gene expression
Materials Chemistry
medicine
Alkaline phosphatase
Bone marrow
Stem cell
TA1-2040
Subjects
Details
- Language :
- English
- ISSN :
- 20796412
- Volume :
- 11
- Issue :
- 642
- Database :
- OpenAIRE
- Journal :
- Coatings
- Accession number :
- edsair.doi.dedup.....0aee619f74533f2ae95ba983c2f0f854