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In vitro evaluation of folate-modified PLGA nanoparticles containing paclitaxel for ovarian cancer therapy
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Ovarian cancer is the most lethal gynecological cancer of female reproductive system. In order to improve the survival rate, some modifications on nanoparticles surfaces have been investigated to promote active targeting of drugs into tumor microenvironment. The aim of this study was the development and characterization of folate-modified (PN-PCX-FA) and unmodified PLGA nanoparticles (PN-PCX) containing paclitaxel for ovarian cancer treatment. Nanocarriers were produced using nanoprecipitation technique and characterized by mean particle diameter (MPD), polydispersity index (PDI), zeta potential (ZP), encapsulation efficiency (EE), DSC, FTIR, in vitro cytotoxicity and cellular uptake. PN-PCX and PN-PCX-FA showed MPD 150 nm and PDI 0.2 with high EE (about 90%). Cytotoxicity assays in SKOV-3 cells demonstrated the ability of both formulations to cause cellular damage. PCX encapsulated in PN-PCX-FA at 1 nM showed higher cytotoxicity than PN-PCX. Folate-modified nanoparticles showed a 3.6-fold higher cellular uptake than unmodified nanoparticles. PN-PCX-FA is a promising system to improve safety and efficacy of ovarian cancer treatment. Further in vivo studies are necessary to prove PN-PCX-FA potential.
- Subjects :
- Boron Compounds
Materials science
Paclitaxel
medicine.medical_treatment
NANOPARTÍCULAS
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
chemistry.chemical_compound
Folic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Cell Line, Tumor
Spectroscopy, Fourier Transform Infrared
medicine
Zeta potential
Humans
Particle Size
Survival rate
Ovarian Neoplasms
Tumor microenvironment
Chemotherapy
Calorimetry, Differential Scanning
Cell Death
021001 nanoscience & nanotechnology
medicine.disease
Endocytosis
In vitro
0104 chemical sciences
chemistry
Mechanics of Materials
Cancer research
Nanoparticles
Female
Nanocarriers
0210 nano-technology
Ovarian cancer
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....0ae99d827f62e23aae3ef1009c94f837