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Susceptibility of BALB/c-nu/nu Mice and BALB/c Mice to Equine Herpesvirus 9 Infection
- Source :
- Veterinary Pathology. 51:581-590
- Publication Year :
- 2013
- Publisher :
- SAGE Publications, 2013.
-
Abstract
- This study aimed to clarify the timing and infectivity of equine herpesvirus 9 (EHV-9) infection in BALB/c-nu/nu mice and their immunocompetent counterpart (BALB/c). Following intranasal inoculation with 105PFU of EHV-9, specimens from 8 mice per group were collected at different times postinoculation (PI) and assessed using histopathology, immunohistochemistry for viral antigen, and quantitative real-time polymerase chain reaction for ORF30 gene expression. In BALB/c-nu/nu mice, EHV-9 antigen was abundant in olfactory epithelia of all inoculated animals, and in the olfactory bulb of 1 animal. In contrast, only 1 BALB/c mouse per time point had rhinitis, with mild to moderate immunopositivity starting from 12 to 48 h PI, followed by a gradual virus clearance at 72 h PI. Statistically, significant differences were noted in the immunohistochemistry reactions between the 2 mouse strains, indicating that BALB/c-nu/nu is more susceptible to infection. Relative expression levels of ORF30 gene in olfactory epithelia were significantly different between the 2 groups, with the exception of 12 h PI, when BALB/c-nu/nu animals showed dramatic increases in ORF30 gene expression level until 48 h PI, followed by a decline in expression level until the end of experiment. In contrast, the expression level in brains showed no differences between mouse strain except at 96 h PI. In both strains, the highest messenger RNA expression was detected at 48 h PI, followed by a decline in BALB/c mice, proving a rapid clearance of virus in BALB/c and a gradual slowing down of the increased expression levels in BALB/c-nu/nu.
- Subjects :
- Mice, Nude
Real-Time Polymerase Chain Reaction
Statistics, Nonparametric
Virus
Cell Line
BALB/c
Rodent Diseases
Mice
Olfactory Mucosa
Antigen
Gene expression
Animals
Varicellovirus
RNA, Messenger
Antigens, Viral
Administration, Intranasal
DNA Primers
Infectivity
Mice, Inbred BALB C
Messenger RNA
General Veterinary
biology
Herpesviridae Infections
biology.organism_classification
Immunohistochemistry
Virology
Molecular biology
Olfactory bulb
Cattle
Disease Susceptibility
Subjects
Details
- ISSN :
- 15442217 and 03009858
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Veterinary Pathology
- Accession number :
- edsair.doi.dedup.....0ae3f915f2ff1bbff44ca842c6ee8a3e