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β-Carotene influences the phycobilisome antenna of cyanobacterium Synechocystis sp. PCC 6803
- Source :
- Photosynthesis Research. 130:403-415
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- We investigated the relation between the carotenoid composition and the structure of phycobilisome (PBS) antenna of cyanobacterium Synechocystis sp. PCC 6803. PBS is a large soluble protein complex enhances the light harvesting efficiency of the cells. It is composed of a central allophycocyanin core and radial phycocyanin rods, but it does not contain carotenoids. However, the absence or low level of carotenoids were previously shown to lead the co-existence of unconnected rod units and assembled PBS with shorter peripheral rods. Here we show that the lack of β-carotene, but not of xanthophylls or the distortion of photosystem structure, evoked unconnected rods. Thus, these essential β-carotene molecules are not bound by Photosystem I or Photosystem II. Our results do not show correlation between the reactive oxygen species (ROS) and PBS distortion despite the higher singlet oxygen producing capacity and light sensitivity of the mutant cells. Reduced cellular level of those linker proteins attaching the rod units together was also observed, but the direct damage of the linkers by ROS are not supported by our data. Enzymatic PBS proteolysis induced by nitrogen starvation in carotenoid mutant cells revealed a retarded degradation of the unconnected rod units.
- Subjects :
- 0301 basic medicine
Light
Photosystem II
Nitrogen
Light-Harvesting Protein Complexes
macromolecular substances
Plant Science
Biology
Photosynthesis
Photosystem I
Biochemistry
03 medical and health sciences
Phycocyanin
Phycobilisomes
polycyclic compounds
Photosystem
Allophycocyanin
Synechocystis
Cell Biology
General Medicine
beta Carotene
biology.organism_classification
Glucose
Spectrometry, Fluorescence
030104 developmental biology
Phycobilisome
Subjects
Details
- ISSN :
- 15735079 and 01668595
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Photosynthesis Research
- Accession number :
- edsair.doi.dedup.....0ae363624273ce7ededd08f30b7e6ffc