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Acrylic Injectable and Self-Curing Formulations for the Local Release of Bisphosphonates in Bone Tissue
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2007
- Publisher :
- Wiley-Blackwell, 2007.
-
Abstract
- Two bisphosphonates (BPs), namely 1-hydroxy-2-[4-aminophenyl]ethane-1,1-diphosphonic acid (APBP) and 1-hydroxy-2-[3-indolyl]ethane-1,1-diphosphonic acid (IBP), have been synthesized and incorporated to acrylic injectable and self-curing formulations. Alendronic acid monosodium trihydrated salt (ALN) containing cement was formulated as control. These systems have potential applications in low density hard tissues affected by ailments characterized by a high osteoclastic resorption, i.e. osteoporosis and osteolysis. Values of curing parameters of APBP and IBP were acceptable to obtain pastes with enough fluency to be injected through a biopsy needle into the bone cavity. Working times ranged between 8 and 15 min and maximum temperature was around 508C. Cured systems stored for a month in synthetic body fluid had compressive strengths between 90 and 96 MPa and modulus between 1.2 and 1.3 GPa, which suggest mechanical stabilization after setting and in the short time. BPs were released in PBS at an initial rate depending on the corresponding chemical structure in the order ALN > APBP > IBP to give final concentrations in PBS of 2.21, 0.44, and 0.19 mol/mL for ALN, APBP, and IBP, respectively. Cytotoxicities of bisphosphonates were evaluated, IC50 values being in the order APBP > ALN > IBP. Absence of cytotoxicity coming from leachables of the cured systems was observed in all cases independently of the BP. An improved cell growth and proliferation for the systems loaded with APBP and IBP compared with that loaded with ALN was observed, as assessed by measuring cell adhesion and proliferation, and total DNA content
- Subjects :
- Osteolysis
Materials science
Acrylic
Chemical structure
Biomedical Engineering
Bone tissue
Bone and Bones
Injections
Biomaterials
Bone remodelling
Materials Testing
medicine
Animals
Humans
Controlled release
Curing (chemistry)
Cells, Cultured
Body fluid
bone cement
Diphosphonates
Alendronic acid
Bone Cements
DNA
Fibroblasts
medicine.disease
Bone cement
osteoporosis
medicine.anatomical_structure
Acrylates
Delayed-Action Preparations
Rabbits
Biomedical engineering
medicine.drug
Nuclear chemistry
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....0ae148a3970a0ab664ca9c31db18ae36