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Screening and Characterization of Multidrug-Resistant Gram-Negative Bacteria from a Remote African Area, São Tomé and Príncipe

Authors :
Nicolas Kieffer
Patrice Nordmann
Laurent Poirel
Patrick Kudyba
Marta Aires-de-Sousa
Source :
Antimicrobial agents and chemotherapy. 62(9)
Publication Year :
2018

Abstract

The occurrence of resistance to last-resort antibiotics was evaluated among Enterobacteriaceae isolates recovered from hospitalized children in a remote African archipelago, São Tomé and Príncipe, where there is limited access to those antibiotics. Fifty patients were screened for colonization by carbapenem-, pan- aminoglycoside-, or polymyxin-resistant Enterobacteriaceae. A total of 36 isolates (including 30 Escherichia coli and 4 Klebsiella pneumoniae) were recovered from 23 patients, including 26 isolates harboring the blaOXA-181 carbapenemase gene, a single isolate harboring the 16S rRNA methylase gene rmtB encoding pan-resistance to aminoglycosides, and 8 isolates coharboring both genes. A single isolate possessed the plasmid-borne colistin resistance gene mcr-1. A high clonal relationship was found for OXA-181-producing E. coli (4 clones), and conversely, three of the four OXA-181-producing K. pneumoniae isolates were clonally unrelated. This study overall showed a high prevalence of resistance to last-resort antibiotics in this country, where no epidemiological data were previously available.

Details

ISSN :
10986596
Volume :
62
Issue :
9
Database :
OpenAIRE
Journal :
Antimicrobial agents and chemotherapy
Accession number :
edsair.doi.dedup.....0ad42cfbab9054e31fffbfae2160176c