Back to Search
Start Over
Bioinformatic Prediction of an tRNASec Gene Nested inside an Elongation Factor SelB Gene in Alphaproteobacteria
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 4605, p 4605 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- In bacteria, selenocysteine (Sec) is incorporated into proteins via the recoding of a particular codon, the UGA stop codon in most cases. Sec-tRNASec is delivered to the ribosome by the Sec-dedicated elongation factor SelB that also recognizes a Sec-insertion sequence element following the codon on the mRNA. Since the excess of SelB may lead to sequestration of Sec-tRNASec under selenium deficiency or oxidative stress, the expression levels of SelB and tRNASec should be regulated. In this bioinformatic study, I analyzed the Rhizobiales SelB species because they were annotated to have a non-canonical C-terminal extension. I found that the open reading frame (ORF) of diverse Alphaproteobacteria selB genes includes an entire tRNASec sequence (selC) and overlaps with the start codon of the downstream ORF. A remnant tRNASec sequence was found in the Sinorhizobium melilotiselB genes whose products have a shorter C-terminal extension. Similar overlapping traits were found in Gammaproteobacteria and Nitrospirae. I hypothesized that once the tRNASec moiety is folded and processed, the expression of the full-length SelB may be repressed. This is the first report on a nested tRNA gene inside a protein ORF in bacteria.
- Subjects :
- 0301 basic medicine
QH301-705.5
selenocysteine
non-coding RNA
Biology
Catalysis
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Start codon
Bacterial Proteins
RNA, Transfer
RNA, Messenger
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Gene
Spectroscopy
Alphaproteobacteria
Genetics
Selenocysteine
Communication
Organic Chemistry
Computational Biology
General Medicine
RNA, Transfer, Amino Acid-Specific
Genetic code
Peptide Elongation Factors
Stop codon
Computer Science Applications
Elongation factor
genetic code
Chemistry
Open reading frame
030104 developmental biology
chemistry
Protein Biosynthesis
Transfer RNA
Codon, Terminator
Nucleic Acid Conformation
Ribosomes
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....0acbd1601ac3ea5b739b6ceeac52b7cc