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Downstream sequence-dependent RNA cleavage and pausing by RNA polymerase I
- Source :
- J Biol Chem
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The sequence of the DNA template has long been thought to influence the rate of transcription by DNA-dependent RNA polymerases, but the influence of DNA sequence on transcription elongation properties of eukaryotic RNA polymerase I (Pol I) fromSaccharomyces cerevisiaehas not been defined. In this study, we observe changes in dinucleotide production, transcription elongation complex stability, and Pol I pausingin vitroin response to downstream DNA.In vitrostudies demonstrate that AT-rich downstream DNA enhances pausing by Pol I and inhibits Pol I nucleolytic cleavage activity. Analysis of Pol I native elongating transcript sequencing data inSaccharomyces cerevisiaesuggests that these downstream sequence elements influence Pol Iin vivo. Native elongating transcript sequencing studies reveal that Pol I occupancy increases as downstream AT content increases and decreases as downstream GC content increases. Collectively, these data demonstrate that the downstream DNA sequence directly impacts the kinetics of transcription elongation prior to the sequence entering the active site of Pol I bothin vivoandin vitro.
- Subjects :
- Transcription Elongation, Genetic
viruses
Oligonucleotides
Saccharomyces cerevisiae
Biochemistry
DNA sequencing
chemistry.chemical_compound
Transcription (biology)
RNA Polymerase I
Transcriptional regulation
RNA polymerase I
DNA, Fungal
Molecular Biology
Polymerase
RNA Cleavage
Base Composition
biology
Base Sequence
Chemistry
RNA
Cell Biology
AT Rich Sequence
Cell biology
Mutation
biology.protein
Enzymology
DNA
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 295
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....0acb642e7a3f28f15c821c078981b35c