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SARS Coronavirus-2 Microneutralisation and Commercial Serological Assays Correlated Closely for Some but Not All Enzyme Immunoassays
- Source :
- Viruses, Volume 13, Issue 2, Viruses, Vol 13, Iss 247, p 247 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Serological testing for SARS-CoV-2-specific antibodies provides important research and diagnostic information relating to COVID-19 prevalence, incidence and host immune response. A greater understanding of the relationship between functionally neutralising antibodies detected using microneutralisation assays and binding antibodies detected using scalable enzyme immunoassays (EIA) is needed in order to address protective immunity post-infection or vaccination, and assess EIA suitability as a surrogate test for screening of convalescent plasma donors. We assessed whether neutralising antibody titres correlated with signal cut-off ratios in five commercially available EIAs, and one in-house assay based on expressed spike protein targets. Sera from recovered patients or convalescent plasma donors who reported laboratory-confirmed SARS-CoV-2 infection (n = 200), and negative control sera collected prior to the COVID-19 pandemic (n = 100), were assessed in parallel. Performance was assessed by calculating EIA sensitivity and specificity with reference to microneutralisation. Neutralising antibodies were detected in 166 (83%) samples. Compared with this, the most sensitive EIAs were the Cobas Elecsys Anti-SARS-CoV-2 (98%) and Vitros Immunodiagnostic Anti-SARS-CoV-2 (100%), which detect total antibody targeting the N and S1 antigens, respectively. The assay with the best quantitative relationship with microneutralisation was the Euroimmun IgG. These results suggest the marker used (total Ab vs. IgG vs. IgA) and the target antigen are important determinants of assay performance. The strong correlation between microneutralisation and some commercially available assays demonstrates their potential for clinical and research use in assessing protection following infection or vaccination, and use as a surrogate test to assess donor suitability for convalescent plasma donation.
- Subjects :
- 0301 basic medicine
Convalescent plasma
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
lcsh:QR1-502
serology
Enzyme-Linked Immunosorbent Assay
Antibodies, Viral
Sensitivity and Specificity
lcsh:Microbiology
Article
Serology
COVID-19 Serological Testing
03 medical and health sciences
0302 clinical medicine
Immune system
Antigen
Neutralization Tests
Virology
Medicine
Humans
030212 general & internal medicine
biology
business.industry
SARS-CoV-2
COVID-19
neutralising antibody
Immunoglobulin A
Vaccination
030104 developmental biology
Infectious Diseases
ROC Curve
Immunoglobulin G
convalescent plasma
biology.protein
Enzyme immunoassays
Antibody
business
0605 Microbiology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Viruses, Volume 13, Issue 2, Viruses, Vol 13, Iss 247, p 247 (2021)
- Accession number :
- edsair.doi.dedup.....0aac3edcfc8b24feab29a9278237b642