Supplemental Figures from TPL2 Is an Oncogenic Driver in Keratocanthoma and Squamous Cell Carcinoma

Supplementary Fig. S1 - Generation of inducible TPL2 transgenic (iTPL2 TG) mice. Supplementary Fig. S2 - Percentage of tumor-free for iTPL2 TG mice ON DOX Supplementary Fig. S3 - Detection of transgene TPL2 expression using an antibody against HA epitope tag (A) and three different antibodies against TPL2 (B) in an iTPL2 wt (#100) TG-driven KA-like cSCC ON DOX. Supplementary Fig. S4 - A, effects on TPL2-related signaling pathways with the treatments of indicated inhibitors in stable cell lines, overexpressing either control vector (Vector), TPL2-WT, or TPL2-IN, by western blot. Supplementary Fig. S5 - Western blot analyses of normal mouse skin samples Supplementary Fig. S6 - For In vitro kinase assay Supplementary Fig. S7 -Co-immunofluorescence of TPL2 Supplementary Fig. S8 - Quantification of each staining with iTPL2 TG-driven cSCC