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Klebsiella pneumoniae Multiresistance Plasmid pMET1: Similarity with the Yersinia pestis Plasmid pCRY and Integrative Conjugative Elements
- Source :
- PLoS ONE, PLoS ONE, Vol 3, Iss 3, p e1800 (2008)
- Publication Year :
- 2008
- Publisher :
- Public Library of Science, 2008.
-
Abstract
- Background Dissemination of antimicrobial resistance genes has become an important public health and biodefense threat. Plasmids are important contributors to the rapid acquisition of antibiotic resistance by pathogenic bacteria. Principal Findings The nucleotide sequence of the Klebsiella pneumoniae multiresistance plasmid pMET1 comprises 41,723 bp and includes Tn1331.2, a transposon that carries the bla TEM-1 gene and a perfect duplication of a 3-kbp region including the aac(6′)-Ib, aadA1, and bla OXA-9 genes. The replication region of pMET1 has been identified. Replication is independent of DNA polymerase I, and the replication region is highly related to that of the cryptic Yersinia pestis 91001 plasmid pCRY. The potential partition region has the general organization known as the parFG locus. The self-transmissible pMET1 plasmid includes a type IV secretion system consisting of proteins that make up the mating pair formation complex (Mpf) and the DNA transfer (Dtr) system. The Mpf is highly related to those in the plasmid pCRY, the mobilizable high-pathogenicity island from E. coli ECOR31 (HPIECOR31), which has been proposed to be an integrative conjugative element (ICE) progenitor of high-pathogenicity islands in other Enterobacteriaceae including Yersinia species, and ICEKp1, an ICE found in a K. pneumoniae strain causing primary liver abscess. The Dtr MobB and MobC proteins are highly related to those of pCRY, but the endonuclease is related to that of plasmid pK245 and has no significant homology with the protein of similar function in pCRY. The region upstream of mobB includes the putative oriT and shares 90% identity with the same region in the HPIECOR31. Conclusions The comparative analyses of pMET1 with pCRY, HPIECOR31, and ICEKp1 show a very active rate of genetic exchanges between Enterobacteriaceae including Yersinia species, which represents a high public health and biodefense threat due to transfer of multiple resistance genes to pathogenic Yersinia strains.
- Subjects :
- Transposable element
Klebsiella pneumoniae
Yersinia pestis
lcsh:Medicine
Microbiology
Infectious Diseases/Bacterial Infections
03 medical and health sciences
Plasmid
Species Specificity
Microbiology/Environmental Microbiology
lcsh:Science
Gene
030304 developmental biology
Genetics
0303 health sciences
Multidisciplinary
Microbiology/Microbial Evolution and Genomics
biology
Infectious Diseases/Antimicrobials and Drug Resistance
030306 microbiology
lcsh:R
DNA replication
Microbiology/Medical Microbiology
Drug Resistance, Microbial
biology.organism_classification
Enterobacteriaceae
Genetics and Genomics/Microbial Evolution and Genomics
3. Good health
Conjugation, Genetic
biology.protein
lcsh:Q
DNA polymerase I
Microbiology/Cellular Microbiology and Pathogenesis
Research Article
Biotechnology
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 3
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....0a9bc03ddf41593a654a32a405d466f6