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Immunodetection of poly(A) binding protein II in the cell nucleus
- Source :
- Experimental cell research. 214(1)
- Publication Year :
- 1994
-
Abstract
- During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microscopy detected PAB II exclusively in the cell nucleus, both in a widespread staining and in more intensely stained "speckles." PAB II was excluded from the nucleoli. By electron microscopy, PAB II was also found almost exclusively in the nucleus, predominantly in clusters of interchromatin granules, likely corresponding to the speckles observed by immunofluorescence microscopy, and in perichromatin fibrils, which represent nascent transcripts and probably the sites of pre-mRNA processing. In addition, electron microscopy also detected PAB II in nucleoli. The distribution corresponds largely to that of other factors involved in the processing of pre-mRNA and is thus in agreement with the proposed role of the protein in polyadenylation.
- Subjects :
- Cytoplasm
Polyadenylation
Nucleolus
Blotting, Western
Fluorescent Antibody Technique
Biology
Poly(A)-Binding Proteins
medicine
Humans
RNA Processing, Post-Transcriptional
Perichromatin fibrils
Cell Nucleus
Binding protein
RNA-Binding Proteins
Cell Biology
Molecular biology
Poly(A)-Binding Protein II
Cell Compartmentation
Cell nucleus
Microscopy, Electron
medicine.anatomical_structure
Microscopy, Fluorescence
Nucleus
Poly(A)-Binding Protein I
Cell Nucleolus
HeLa Cells
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 214
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....0a902178b696ca241570925a9b8d6ea1