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Protective effects of the angiotensin type 1 receptor antagonist losartan in infection-induced and arthritis-associated alveolar bone loss
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Europe PubMed Central
- Publication Year :
- 2015
-
Abstract
- Background and Objective The angiotensin type 1 (AT1) receptor has been implicated in the pathogenesis of inflammatory bone disorders. This study aimed to investigate the effect of an AT1 receptor antagonist in infection-induced and arthritis-associated alveolar bone loss in mice. Material and Methods Mice were subjected to Aggregatibacter actinomycetemcomitans oral infection or antigen-induced arthritis and treated daily with 10 mg/kg of the prototype AT1 antagonist, losartan. Treatment was conducted for 30 d in the infectious condition and for 17 d and 11 d in the preventive or therapeutic regimens in the arthritic model, respectively. The mice were then killed, and the maxillae, serum and knee joints were collected for histomorphometric and immunoenzymatic assays. In vitro osteoclast assays were performed using RAW 264.7 cells stimulated with A. actinomycetemcomitans lipopolysacharide (LPS). Results Arthritis and A. actinomycetemcomitans infection triggered significant alveolar bone loss in mice and increased the levels of myeloperoxidase and of TRAP+ osteoclasts in periodontal tissues. Losartan abolished such a phenotype, as well as the arthritis joint inflammation. Both arthritis and A. actinomycetemcomitans conditions were associated with the release of tumor necrosis factor alpha (TNF-α), interferon-gamma, interleukin-17 and chemokine (C-X-C motif) ligand 1 and an increased RANKL/osteoprotegerin ratio in periodontal tissues, but such expression decreased after losartan treatment, except for TNF-α. The therapeutic approach was as beneficial as the preventive one. In vitro, losartan prevented LPS-induced osteoclast differentiation and activity. Conclusion The blockade of AT1 receptor exerts anti-inflammatory and anti-osteoclastic effects, thus protecting periodontal tissues in distinct pathophysiological conditions of alveolar bone loss.
- Subjects :
- Male
Serum
musculoskeletal diseases
medicine.medical_specialty
Knee Joint
medicine.drug_class
Alveolar Bone Loss
Anti-Inflammatory Agents
Arthritis
Aggregatibacter actinomycetemcomitans
Losartan
Receptor, Angiotensin, Type 1
OSTEOBLASTO
Mice
Osteoprotegerin
Osteoclast
Internal medicine
Maxilla
medicine
Animals
Dental alveolus
Mice, Inbred BALB C
Angiotensin II receptor type 1
biology
Histocytochemistry
business.industry
medicine.disease
Receptor antagonist
Mice, Inbred C57BL
RAW 264.7 Cells
Endocrinology
medicine.anatomical_structure
RANKL
biology.protein
Periodontics
Pasteurellaceae Infections
business
medicine.drug
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....0a877dafa2fa5ae418910d3238107a53