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Development of a BoHV-4 viral vector expressing tgD of BoHV-1 and evaluation of its immunogenicity in mouse model
- Source :
- Brazilian Journal of Microbiology
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- In recent years, Bovine herpesvirus 4 (BoHV-4) has emerged as an attractive gene delivery viral vector, mainly for vaccination purposes in the veterinary field. In the present study, a new infectious clone of the BoHV-4 genome carrying a bacterial artificial chromosome vector (BoHV-4-BAC) was developed by homologous recombination in mammalian cell culture and bacterial systems, and exploited to express a truncated form of glycoprotein D (tgD) of Bovine herpesvirus 1 (BoHV-1) (BoHV-4-tgD∆TK) as a vaccine candidate. This construct's immunogenicity was compared to a DNA vector expressing the same antigen (pC-tgD) in a BALB/c mouse model. After the mice were immunized, total and specific antibody responses, cytokine responses, total splenocyte cells proliferation/cytotoxicity, and virus neutralization assays were conducted to analyze the immune response elicited by both constructs. Mice from both vaccine groups developed significant humoral and cellular immune responses after a booster dose regime was conducted on day 28 post-injection. In almost all immunological assays, BoHV-4-tgDΔTK induced as high an immune response as pC-tgD. In both vaccine constructs, neutralizing antibodies were a significant determining factor in protection against BoHV-1, even after the first injection. We conclude that a BoHV-4-based viral vector offers an effective immunization strategy as an alternative to DNA-based immunization platforms, at least to combat BoHV-1.
- Subjects :
- Booster dose
Bacterial, Fungal and Virus Molecular Biology - Research Paper
TgD
Antibodies, Viral
Microbiology
Viral vector
Mice
Viral Proteins
Immune system
Antigen
Bovine herpesvirus 1
Media Technology
Animals
Bovine herpesvirus 4
Immune response
Herpesvirus 1, Bovine
Mice, Inbred BALB C
Bacterial artificial chromosome
biology
Immunogenicity
biology.organism_classification
Antibodies, Neutralizing
Virology
Herpesvirus 4, Bovine
biology.protein
Antibody
Subjects
Details
- ISSN :
- 16784405 and 15178382
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....0a877a8f1e912609cbe3a6169a8f6027