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Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples
- Source :
- Food Technology and Biotechnology, Vol 57, Iss 1, Pp 97-104 (2019), Food Technology and Biotechnology
- Publication Year :
- 2019
- Publisher :
- University of Zagreb, 2019.
-
Abstract
- Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair.
- Subjects :
- General Chemical Engineering
lcsh:Biotechnology
fermented sausages
Industrial and Manufacturing Engineering
law.invention
Starter
law
Lactobacillus
lcsh:TP248.13-248.65
Multiplex
Food science
Polymerase chain reaction
biology
lcsh:TP368-456
starter cultures
Lactobacillus sakei
food and beverages
biology.organism_classification
lcsh:Food processing and manufacture
Real-time polymerase chain reaction
Lactobacillus curvatus
Fermentation
Preliminary Communications
Primer (molecular biology)
real-time PCR
HRM
Food Science
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 13342606 and 13309862
- Volume :
- 57
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Food Technology and Biotechnology
- Accession number :
- edsair.doi.dedup.....0a865613c599a2ba833e5a4e0c5c4702