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Evidence for ammonium conductance in a mouse thick ascending limb cell line
- Source :
- Physiological Reports
- Publication Year :
- 2017
- Publisher :
- John Wiley and Sons Inc., 2017.
-
Abstract
- In this study, we examined an ammonium conductance in the mouse thick ascending limb cell line ST‐1. Whole cell patch clamp was performed to measure currents evoked by NH 4Cl in the presence of BaCl2, tetraethylammonium, and BAPTA. Application of 20 mmol/L NH 4Cl induced an inward current (−272 ± 79 pA, n = 9). In current‐voltage (I–V) relationships, NH 4Cl application caused the I–V curve to shift down in an inward direction. The difference in current before and after NH 4Cl application, which corresponds to the current evoked by NH 4Cl, was progressively larger at more negative potentials. The reversal potential for NH4Cl was +15 mV, higher than the equilibrium potential for chloride, indicating that the current should be due to NH 4 +. We then injected ST‐1 poly(A) RNA into Xenopus oocytes and performed two‐electrode voltage clamp. NH 4Cl application in the presence of BaCl2 caused the I–V curve to be steeper. The NH 4 + current was retained at pH 6.4, where endogenous oocyte current was abolished. The NH 4 + current was unaffected by 10 μmol/L amiloride but abolished after incubation in Na+‐free media. These results demonstrate that the renal cell line ST‐1 produces an NH 4 + conductance.
- Subjects :
- inorganic chemicals
0301 basic medicine
medicine.medical_specialty
Physiology
Voltage clamp
Xenopus
Action Potentials
Kidney
Cell Line
Amiloride
03 medical and health sciences
chemistry.chemical_compound
Mice
BAPTA
Chlorides
Physiology (medical)
Internal medicine
Membrane Physiology
Ammonium Compounds
medicine
Ammonium conductance
Animals
Ammonium
Renal Filtration
Patch clamp
Reversal potential
Diuretics
Egtazic Acid
Original Research
Xenopus oocytes
Tetraethylammonium
Ion Transport
Conductance
Epithelial Cells
030104 developmental biology
Endocrinology
chemistry
thick ascending limb
Barium
Biophysics
Loop of Henle
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 2051817X
- Volume :
- 5
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Physiological Reports
- Accession number :
- edsair.doi.dedup.....0a81d055ce7224dbaf8fc20726d62883