Back to Search
Start Over
Simple and sensitive liquid chromatography–tandem mass spectrometry method for determination of the S(+)- and R(−)-enantiomers of baclofen in human plasma and cerebrospinal fluid
- Source :
- Journal of Chromatography B. 801:257-264
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- A simple and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method to determine the enantiomers of the muscle relaxant baclofen in human plasma and cerebrospinal fluid (CSF) has been developed. A commercially available ultrafiltration membrane is used to prepare the sample. A chiral CROWNPAK CR(+) stationary phase column is then used to perform complete resolution of the S(+)- and R(-)-enantiomers of baclofen. This method was used to analyze human plasma and CSF spiked with baclofen, and the calibration curves for both biologic samples were linear over a concentration range of 0.15-150 ng enantiomer/ml. The lower limit of quantification was 0.15 ng enantiomer/ml in both fluids. Finally, the method was tested with an artificial CSF as an alternative to authentic human CSF. The results showed that no matrix effects and no interfering peaks were observed using this artificial CSF.
- Subjects :
- Baclofen
Chromatography
Muscle Relaxants, Central
Chemistry
Calibration curve
Clinical Biochemistry
Ultrafiltration
Stereoisomerism
Cell Biology
General Medicine
Mass spectrometry
Sensitivity and Specificity
Biochemistry
Mass Spectrometry
Specimen Handling
Analytical Chemistry
Solutions
Cerebrospinal fluid
Liquid chromatography–mass spectrometry
Blood plasma
Humans
Enantiomer
Quantitative analysis (chemistry)
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 801
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....0a80f3b624511ae37d49b1cb9536742c
- Full Text :
- https://doi.org/10.1016/j.jchromb.2003.11.025