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Problems with Fixation and Embedding of Arbuscular Mycorrhizal Fungi (Glomales)
- Source :
- Mycologia. 85:323-330
- Publication Year :
- 1993
- Publisher :
- Informa UK Limited, 1993.
-
Abstract
- In order to conduct ultrastructural studies to clarify the nature of the spores formed in Glomales, an order of arbuscular mycorrhizal fungi, techniques for fixation and embedding of these spores were tested. Of nine methods, none was effective for fixation of Acaulospora, Gigaspora, and Scutellospora spores, but one was efficient for fixation of Glomus. This method included fixation with glutaraldehyde in a microwave oven, and breaking the spores under liquid nitrogen before postfixation with osmium tetroxide. For spore walls, however, most of the techniques were satisfactory for all of the genera tested.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Fixation (surgical)
Botany
Genetics
Acaulospora
Mycorrhiza
Phycomycetes
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Glomus
biology
fungi
Cell Biology
General Medicine
030108 mycology & parasitology
biology.organism_classification
Spore
Osmium tetroxide
chemistry
Glutaraldehyde
Subjects
Details
- ISSN :
- 15572536 and 00275514
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Mycologia
- Accession number :
- edsair.doi.dedup.....0a518df23524f92573f0b0916c458c97