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Selective measurement of NAPE-PLD activity via a PLA1/2-resistant fluorogenic N-acyl-phosphatidylethanolamine analog
- Source :
- Journal of Lipid Research, Vol 63, Iss 1, Pp 100156-(2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a zinc metallohydrolase enzyme that converts NAPEs to bioactive N-acyl-ethanolamides (NAEs). Altered NAPE-PLD activity may contribute to pathogenesis of obesity, diabetes, atherosclerosis, and neurological diseases. Selective measurement of NAPE-PLD activity is challenging, however, due to alternative phospholipase pathways for NAPE hydrolysis. Previous methods to measure NAPE-PLD activity involved addition of exogenous NAPE followed by thin-layer chromatography (TLC) or liquid chromatography-tandem mass spectrometry (LC/MS/MS), which are time- and resource-intensive. Recently, NAPE-PLD activity in cells has been assayed using the fluorogenic NAPE analogs PED-A1 and PED6, but these substrates also detect the activity of serine hydrolase-type lipases PLA1 and PLA2. To create a fluorescence assay that selectively measured cellular NAPE-PLD activity, we synthesized an analog of PED-A1 (flame-NAPE) where the sn-1 ester bond was replaced with a N-methyl amide to create resistance to PLA1 hydrolysis. Recombinant NAPE-PLD produced fluorescence when incubated with either PED-A1 or flame-NAPE, while PLA1 only produced fluorescence when incubated with PED-A1. Furthermore, fluorescence in HepG2 cells using PED-A1 could be partially blocked by either biothionol (a selective NAPE-PLD inhibitor) or tetrahydrolipstatin (an inhibitor of a broad spectrum of serine hydrolase-type lipases). In contrast, fluorescence assayed in HepG2 cells using flame-NAPE could only be blocked by biothionol. In multiple cell types, the phospholipase activity detected using flame-NAPE was significantly more sensitive to biothionol inhibition than that detected using PED-A1. Thus, using flame-NAPE to measure phospholipase activity provides a rapid and selective method to measure NAPE-PLD activity in cells and tissues.
- Subjects :
- Nape
QD415-436
NAPE-PLD
N-acylphosphatidylethanolamide
Phospholipase
Biochemistry
law.invention
Serine
chemistry.chemical_compound
Endocrinology
Phospholipase A1
law
medicine
chemistry.chemical_classification
Phosphatidylethanolamine
biothionol
Chemistry
Phospholipase D
Cell Biology
N-acylethanolamide
Enzyme
medicine.anatomical_structure
Recombinant DNA
phospholipase A1
lipids (amino acids, peptides, and proteins)
fluorescence
Subjects
Details
- ISSN :
- 00222275
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....0a45ec12ab076a74b1118dd3bbbe2626