Orthos hyalinus, new status

Orthos hyalinus (E. Bell, 1930), reinstated status and Orthos minka Evans, 1955, new status Genomic sequencing and comparison of Eutychide hyalinus Bell, 1930 (type locality in Brazil: Santa Catarina, holotype NVG-18026A06 sequenced) and Orthos orthos minka Evans, 1955 (type locality Brazil: Amazonas, Manaus), currently subspecies of Eutychide orthos Godman, 1900 (type locality Panama: Chiriqui), which is the type species of Orthos Evans, 1955, reveal prominent genetic differentiation among them (Fig. 4 magenta, olive and green). E.g., COI barcodes of E. hyalinus and O. o. minka respectively differ from O. orthos by 5.9% (39 bp) and 8.3% (55 bp). The genetic differentiation of O. o. minka is especially large, near the levels characteristic of subgenera (Fig. 4). Taken together with phenotypic differences described by Evans (1955), who particularly distinguished O. o. minka by the shape of brands and unmarked wings, the genetic differences support species status for these taxa: Orthos hyalinus (E. Bell, 1930), stat. rest. and Orthos minka Evans, 1955, stat. nov. Genitalia of the three species differ as well, in the shape of harpe and the extent of its separation from ampulla, although not described, but illustrated by Evans (1955).
Published as part of Zhang, Jing, Dolibaina, Diego R., Cong, Qian, Shen, Jinhui, Song, Leina, Mielke, Carlos G. C., Casagrande, Mirna M., Mielke, Olaf H. H. & Grishin, Nick V., 2023, Taxonomic notes on Neotropical Hesperiidae (Lepidoptera), pp. 91-114 in Zootaxa 5271 (1) on page 98, DOI: 10.11646/zootaxa.5271.1.3, http://zenodo.org/record/7864266
{"references":["Evans, W. H. (1955) A catalogue of the American Hesperiidae indicating the classification and nomenclature adopted in the British Museum (Natural History). Part IV. Hesperiinae and Megathyminae. The Trustees of the British Museum (Natural History), London, v + 499 pp., pls. 454 - 488."]}