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Age and Sex-Dependent ADNP Regulation of Muscle Gene Expression Is Correlated with Motor Behavior: Possible Feedback Mechanism with PACAP
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 18
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The activity-dependent neuroprotective protein (ADNP), a double-edged sword, sex-dependently regulates multiple genes and was previously associated with the control of early muscle development and aging. Here we aimed to decipher the involvement of ADNP in versatile muscle gene expression patterns in correlation with motor function throughout life. Using quantitative RT-PCR we showed that Adnp+/&minus<br />heterozygous deficiency in mice resulted in aberrant gastrocnemius (GC) muscle, tongue and bladder gene expression, which was corrected by the Adnp snippet, drug candidate, NAP (CP201). A significant sexual dichotomy was discovered, coupled to muscle and age-specific gene regulation. As such, Adnp was shown to regulate myosin light chain (Myl) in the gastrocnemius (GC) muscle, the language acquisition gene forkhead box protein P2 (Foxp2) in the tongue and the pituitary-adenylate cyclase activating polypeptide (PACAP) receptor PAC1 mRNA (Adcyap1r1) in the bladder, with PACAP linked to bladder function. A tight age regulation was observed, coupled to an extensive correlation to muscle function (gait analysis), placing ADNP as a muscle-regulating gene/protein.
- Subjects :
- Male
Myosin Light Chains
Myosin light-chain kinase
Urinary Bladder
Gene Expression
Nerve Tissue Proteins
Motor Activity
Biology
Article
Catalysis
Feedback
Inorganic Chemistry
Mice
Tongue
CatWalk
Gene expression
Animals
RNA, Messenger
Physical and Theoretical Chemistry
Muscle, Skeletal
Receptor
Molecular Biology
Gene
ADCYAP1R1
ADNP
Spectroscopy
Homeodomain Proteins
Regulation of gene expression
Messenger RNA
muscle function
Organic Chemistry
Forkhead Transcription Factors
FOXP2
General Medicine
Computer Science Applications
Cell biology
Mice, Inbred C57BL
NAP
Gene Expression Regulation
Pituitary Adenylate Cyclase-Activating Polypeptide
Female
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....0a2758e8e9c31abbc412072f100d14e8