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Differential expression of neural cell adhesion molecule (NCAM) after tenotomy in rabbit skeletal muscle
- Source :
- Journal of Orthopaedic Research. 20:364-369
- Publication Year :
- 2002
- Publisher :
- Wiley, 2002.
-
Abstract
- Tenotomy is a commonly encountered event in orthopaedic surgery. In 23 rabbit extensor digitorum longus (EDL) muscles, within 24 h after tenotomy, a marked drop in maximum force production occurred. This was not explainable based on architectural changes and histological examination using standard markers for muscle injury, i.e., haematoxylin and eosin morphology, developmental myosin heavy chain (MHC) immunolabeling, and quantitation of muscle fiber type percentage, area and distribution. The expression of neural cell adhesion molecule (NCAM), a glycoprotein expressed during muscle development was measured as a function of time in these muscles. NCAM expression was increased as early as one day after tenotomy with 2:2 � 1:2% of the fibers showing positive expression. This expression level increased significantly to 15:4 � 15:2% after 7 days and then subsided to 13:2 � 10:6% 21 days after tenotomy. Two-way analysis of variance demonstrated a significant effect of time and a significant time x tenotomy method interaction. These results suggest that tenotomy leads to possible changes in muscle–nerve connections and/or excitation–contraction (EC) coupling. 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
Tenotomy
Haematoxylin
Injections, Intramuscular
Tendons
chemistry.chemical_compound
Immunolabeling
Tendon Injuries
Isometric Contraction
Internal medicine
Myosin
medicine
Animals
Protein Isoforms
Orthopedics and Sports Medicine
Muscle, Skeletal
Neural Cell Adhesion Molecules
Myosin Heavy Chains
Eosin
Skeletal muscle
Anatomy
Muscle injury
Bupivacaine
Immunohistochemistry
Endocrinology
medicine.anatomical_structure
chemistry
Neural cell adhesion molecule
Rabbits
Subjects
Details
- ISSN :
- 1554527X and 07360266
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Orthopaedic Research
- Accession number :
- edsair.doi.dedup.....0a2138400b079c832f42aa11da7e1fa3