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αIIb Integrin Expression during Development of the Murine Hemopoietic System
- Source :
- Developmental Biology. 243:301-311
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- Integrin alphaIIb is a cell adhesion molecule expressed in association with beta3 by cells of the megakaryocytic lineage, from committed progenitors to platelets. While it is clear that lymphohemopoietic cells differentiating along other lineages do not express this molecule, it has been questioned whether mammalian hemopoietic stem cells (HSC) and various progenitor cells express it. In this study, we detected alphaIIb expression in midgestation embryo in sites of HSC generation, such as the yolk sac blood islands and the hemopoietic clusters lining the walls of the major arteries, and in sites of HSC migration, such as the fetal liver. Since c-Kit, which plays an essential role in the early stages of hemopoiesis, is expressed by HSC, we studied the expression of the alphaIIb antigen in the c-Kit-positive population from fetal liver and adult bone marrow differentiating in vitro and in vivo into erythromyeloid and lymphocyte lineages. Erythroid and myeloid progenitor activities were found in vitro in the c-Kit(+)alphaIIb(+) cell populations from both origins. On the other hand, a T cell developmental potential has never been considered for c-Kit(+)alphaIIb(+) progenitors, except in the avian model. Using organ cultures of embryonic thymus followed by grafting into athymic nude recipients, we demonstrate herein that populations from murine fetal liver and adult bone marrow contain T lymphocyte progenitors. Migration and maturation of T cells occurred, as shown by the development of both CD4(+)CD8- and CD4-CD8(+) peripheral T cells. Multilineage differentiation, including the B lymphoid lineage, of c-Kit(+)alphaIIb(+) progenitor cells was also shown in vivo in an assay using lethally irradiated congenic recipients. Taken together, these data demonstrate that murine c-Kit(+)alphaIIb(+) progenitor cells have several lineage potentialities since erythroid, myeloid, and lymphoid lineages can be generated.
- Subjects :
- Fetal Proteins
Male
Myeloid
Cellular differentiation
migration
Mice
thymus
Bone Marrow
Cell Movement
T-Lymphocyte Subsets
lymphoid and myeloid progenitors
Myeloid Cells
Lymphocytes
Bone Marrow Transplantation
Yolk Sac
Erythroid Precursor Cells
Mice, Inbred BALB C
adult bone marrow
Graft Survival
Gene Expression Regulation, Developmental
Cell Differentiation
Cell biology
Proto-Oncogene Proteins c-kit
Haematopoiesis
medicine.anatomical_structure
Liver
Radiation Chimera
Female
Stem cell
integrin
Hematopoietic System
T cell
B-Lymphocyte Subsets
Bone Marrow Cells
Platelet Glycoprotein GPIIb-IIIa Complex
Thymus Gland
Biology
Colony-Forming Units Assay
Organ Culture Techniques
medicine
Animals
Cell Lineage
Progenitor cell
Molecular Biology
mouse embryo
Cell Biology
Hematopoietic Stem Cells
Embryonic stem cell
Hematopoiesis
Mice, Inbred C57BL
Immunology
Bone marrow
fetal liver
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 243
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....0a1fd0a73f26c833ad69548e34b69d51