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Hindlimb-unloading suppresses B cell population in the bone marrow and peripheral circulation associated with OPN expression in circulating blood cells
- Source :
- Journal of Bone and Mineral Metabolism. 33:48-54
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Rodent hindlimb unloading (HU) by tail-suspension is a model to investigate disuse-induced bone loss in vivo. Previously, we have shown that osteopontin (OPN, also known as Spp1) is required for unloading-induced bone loss. However, how unloading affects OPN expression in the body is not fully understood. Here, we examined OPN expression in peripheral blood of mice subjected to HU. Real-time RT-PCR analysis indicated that OPN expression is increased in circulating peripheral blood cells. This HU-induced increase in OPN mRNA expression was specific in circulating peripheral blood cells, as OPN was not increased in the blood cells in bone marrow. HU-induced enhancement in OPN expression in peripheral blood cells was associated with an increase in the fraction of monocyte/macrophage lineage cells in the peripheral blood. In contrast, HU decreased the fraction size of B-lymphocytes in the peripheral blood. We further examined if B-lymphogenesis is affected in the mice deficient for osteopontin subjected to HU. In bone marrow, HU decreased the population of the B-lymphocyte lineage cells significantly, whereas it did not alter the population of monocyte/macrophage lineage cells. HU also increased the cells in T-lymphocyte lineage in bone marrow. Interestingly, these changes were observed similarly both in OPN-deficient and wild-type mice. These results indicate for the first time that HU increases OPN expression in circulating cells and suppresses bone marrow B-lymphogenesis.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Endocrinology, Diabetes and Metabolism
Population
Osteoclasts
Bone Marrow Cells
Hindlimb
Biology
Peripheral blood mononuclear cell
Bone and Bones
Monocytes
Mice
Imaging, Three-Dimensional
Endocrinology
stomatognathic system
Bone Marrow
Internal medicine
medicine
Animals
Macrophage
Cell Lineage
Orthopedics and Sports Medicine
RNA, Messenger
Osteopontin
Bone Resorption
education
B cell
B-Lymphocytes
education.field_of_study
Macrophages
Monocyte
X-Ray Microtomography
General Medicine
Flow Cytometry
Hematopoietic Stem Cells
Mice, Inbred C57BL
medicine.anatomical_structure
Hindlimb Suspension
Leukocytes, Mononuclear
biology.protein
Bone marrow
Signal Transduction
Subjects
Details
- ISSN :
- 14355604 and 09148779
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Journal of Bone and Mineral Metabolism
- Accession number :
- edsair.doi.dedup.....0a1da5995088f7e617b5eb92d6c46baf