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Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation

Authors :
Sang-Yull Lee
Lloyd Old
Jin-Hyung Park
Young Min Park
Myung-Ha Song
Chang Hun Lee
Min-Ki Lee
Source :
Biotechnology letters. 33(6)
Publication Year :
2010

Abstract

The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various cancer cell lines showed that its expression was related to methylation of the promoter region. Treatment of human cancer cell lines with the demethylating agent 5-aza-2′-deoxycytidine activated the expression of the NY-SAR-35 gene. In addition, transfection experiments on various fragments of the CpG-rich gene promoter indicate that in vitro methylation of the NY-SAR-35 gene promoter results in the loss of promoter activity. The expression of NY-SAR-35 is therefore activated by hypomethylation of the CpG island in the gene promoter.

Details

ISSN :
15736776
Volume :
33
Issue :
6
Database :
OpenAIRE
Journal :
Biotechnology letters
Accession number :
edsair.doi.dedup.....09fb3c0830fcdd3c8b90a8a03a3f04ac