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Impact of processing method on donated human breast milk microRNA content
- Source :
- PLoS ONE, Vol 15, Iss 7, p e0236126 (2020), PLoS ONE, PLOS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Pasteurization of donated human milk preserves it for storage and makes it safe for feeding, but at the expense of its composition, nutritional values and functions. Here, we aimed to investigate the impact of Holder Pasteurization (HoP) and High Pressure Processing (HPP) methods on miRNA in human milk and to evaluate impact of these changes on miRNA functions. Milk samples obtained from women in 50th day of lactation (n = 3) were subjected either to HoP, HPP or remained unpasteurized as a control. Subsequently, miRNA was isolated from whole material and exosomal fraction and sequenced with Illumina NextSeq 500. Sequencing data were processed, read counts were mapped to miRNA and analyzed both quantitatively with DESeq2 and functionally with DIANA mirPath v.3. While HPP caused statistically insignificant decrease in number of miRNA reads compared to unprocessed material, HoP led to 82-fold decrease in whole material (p = 0.0288) and 302-fold decrease in exosomes (p = 0.0021) not leaving enough reads for further analysis. Changes in composition of miRNA fraction before and after HPP indicated uneven stability of individual miRNAs under high pressure conditions, with miR-30d-5p identified as relatively stable and miR-29 family as sensitive to HPP. Interestingly, about 2/3 of unprocessed milk miRNA content consists of only 10 distinct miRNAs with miR-148a-3p at the top. Functional analysis of most abundant human milk miRNAs showed their involvement in signaling pathways, cell communication, proliferation and metabolism that are obviously important in rapidly growing infants. Functions of miRNAs which suffered the greatest depletion during HPP were similar to roles of the majority of unprocessed human milk's miRNA, which indicates that those functions may be weakened although not completely lost. Our findings indicate that HPP is less detrimental to human milk miRNAs than HoP and should be considered in further research on recommended processing procedures for human milk banks.
- Subjects :
- 0301 basic medicine
Physiology
Pasteurization
Exosomes
Biochemistry
law.invention
law
Lactation
Medicine and Health Sciences
Food science
DNA libraries
Breast Milk
2. Zero hunger
Multidisciplinary
Physics
Classical Mechanics
Genomics
Body Fluids
Nucleic acids
medicine.anatomical_structure
Milk
Physical Sciences
Medicine
Female
Anatomy
Cellular Structures and Organelles
Research Article
Adult
Science
Breast milk
Biology
Human Genomics
Hop (networking)
Specimen Handling
Beverages
03 medical and health sciences
Young Adult
microRNA
medicine
Pressure
Genetics
Humans
Vesicles
Milk Banks
Non-coding RNA
Nutrition
Natural antisense transcripts
030109 nutrition & dietetics
Milk, Human
Biology and life sciences
Gene Expression Profiling
Neonates
Cell Biology
DNA
Microvesicles
Gene regulation
Diet
Gene expression profiling
MicroRNAs
030104 developmental biology
RNA
Gene expression
High Pressure
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....09f725f63a8c1d140eb86c7ad3f50d94