Additional file 1 of CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in Huntington’s disease

Additional file 1. Depletion of MSN marker expression does not reflect neuronal loss in zQ175 mice. a, Representative images of EM48 immunostaining in the dorsal striatum and cortex of zQ175 at 3, 6, 12 and 22 months (n=6 mice/genotype, 6-9 images averaged/mouse). b, Quantification of the number of EM48 aggregates in the cortex of zQ175 and its comparison with striatum. c, Representative images of NeuN immunostaining in the dorsal striatum of WT and zQ175 at 3, 6, 12 and 22 months (n=3 mice/genotype). d, e, Representative images of Ctip2 immunostaining in the dorsal striatum of 12 months old WT and zQ175 and quantitative analysis of Ctip2+ cells (n = 3 WT; 3 zQ175, 9 images averaged/mouse). Scale bar, 50 µm. DAPI is used for nuclear staining. f, Cresyl violet staining in the dorsal striatum of 22 months old WT and zQ175 mice. Magnified image represents neurons (white arrow) and glial (black arrow) cells. g, Quantification of neurons from cresyl violet analyses at 12 and h, 22 months old WT and zQ175 mice (3 mice/genotype, 3 images averaged/mouse). i, Dotted line on magnetic resonance images displays the manually traced striatum region of representative mice of each genotype at 22 months of age. j, Striatum volume analyzed in 22-month-old mice from images in G (n=4 mice/genotype, at least 3 images averaged/mouse). Error bars denote mean ± SD. Student’s t-test, p-values