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Automated Electron-Capture Gas Chromatographic Analysis of Flunitrazepam in Plasma
- Source :
- Pharmacology. 24:82-87
- Publication Year :
- 1982
- Publisher :
- S. Karger AG, 1982.
-
Abstract
- Nanogram quantities of flunitrazepam and its major metabolite, desmethylflunitrazepam, can be reliably quantitated in human plasma using electron-capture gas-liquid chromatography. After addition of methylnitrazepam as the internal standard, plasma samples are extracted with an organic solvent (benzene:isoamyl alcohol). The organic extract is separated, evaporated to dryness, reconstituted, and injected onto the chromatograph using an automatic sampling system which allows up to 100 analyses/24 h. The sensitivity limits are 0.5 ng/ml for flunitrazepam and 1-2 ng/ml for desmethylflunitrazepam. Use of the method is illustrated in a study of the pharmacokinetic properties of flunitrazepam following a single 1.0-mg intravenous dose.
Details
- ISSN :
- 14230313 and 00317012
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Pharmacology
- Accession number :
- edsair.doi.dedup.....09ca19639f60c2f1ed922ea400ba2428