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Mouse neutrophils express functional umami taste receptor T1R1/T1R3
- Source :
- BMB Reports, BMB REPORTS(47): 11
- Publication Year :
- 2014
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2014.
-
Abstract
- Neutrophils play an important role in the initiation of innate immunity against infection and injury. Although many different types of G-protein coupled receptors are functionally expressed in neutrophils, no reports have demonstrated functional expression of umami taste receptor in these cells. We observed that mouse neutrophils express the umami taste receptor T1R1/T1R3 through RNA sequencing and quantitative RT-PCR analysis. Stimulation of mouse neutrophils with L-alanine or L-serine, which are ligands for the umami taste receptor, elicited not only ERK or p38 MAPK phosphorylation but also chemotactic migration. Moreover, addition of L-alanine or L-serine markedly reduced the production of several cytokines including TNF-α induced by lipopolysaccharide (LPS) through inhibition of NF-κB activity or STAT3 phosphorylation in neutrophils. Our findings demonstrate that neutrophils express the umami taste receptor, through which tastants stimulate neutrophils, resulting in chemotactic migration, and attenuation of LPS-induced inflammatory response. [BMB Reports 2014; 47(11): 649-654]
- Subjects :
- Lipopolysaccharides
STAT3 Transcription Factor
MAPK/ERK pathway
Umami taste receptor T1R1/T1R3
Neutrophils
Umami
Biology
Biochemistry
Receptors, G-Protein-Coupled
Mice
Chemotaxis
Cytokine
Inflammation
Neutrophil
Umami taste receptorT1R1/T1R3
Cell Movement
Taste receptor
Serine
Animals
Phosphorylation
Receptor
Molecular Biology
Research Articles
Alanine
Innate immune system
Sequence Analysis, RNA
Tumor Necrosis Factor-alpha
NF-kappa B
General Medicine
Cell biology
Gene Expression Regulation
Immunology
Cytokines
RNA
Calcium
Tumor necrosis factor alpha
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....09be037413ce8f6a47ebb42560590ad1