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Quercetin protects radiation-induced DNA damage and apoptosis in kidney and bladder tissues of rats
- Source :
- Free Radical Research. 48:1247-1255
- Publication Year :
- 2014
- Publisher :
- Informa UK Limited, 2014.
-
Abstract
- WOS: 000341231200011<br />PubMed ID: 25039564<br />Ionizing radiation (IR) can induce cell damage and cell death through the reactive oxygen species generated by radiolytic hydrolysis. The present study was aimed to determine the possible protective effects of quercetin, a well-known antioxidant agent, against IR-induced bladder and kidney damage in rats. Sprague-Dawley rats were exposed to 8-Gy whole-abdominal IR and given either vehicle or quercetin (20 mg/kg, ip). Rats were decapitated at either 36 h or 10 days following IR, where quercetin or vehicle injections were repeated once daily, and kidney and bladder samples were obtained for the determination of myeloperoxidase and caspase-3 activities, an index of tissue neutrophil infiltration and apoptosis, respectively. Radiation-induced inflammation was evaluated through tissue cytokine, TNF-alpha levels. In order to examine oxidative DNA damage, tissue 8-hydroxydeoxyguanosine (8-OHdG) levels were measured. All tissues were also examined microscopically. In the saline-treated irradiation groups, myeloperoxidase and caspase-3 activities, 8-OHdG and TNF-alpha levels were found to be increased in both tissues (p < 0.05). In the quercetin-treated-IR groups, all these oxidant responses were prevented significantly (p < 0.05). The present data demonstrate that quercetin, through its free radical scavenging and antioxidant properties, attenuates irradiation-induced oxidative organ injury, suggesting that quercetin may have a potential benefit in radiotherapy by minimizing the adverse effects and will improve patient care.
- Subjects :
- Antioxidant
medicine.medical_treatment
Blotting, Western
Urinary Bladder
Apoptosis
Radiation-Protective Agents
Pharmacology
Kidney
medicine.disease_cause
Biochemistry
Antioxidants
quercetin
Rats, Sprague-Dawley
chemistry.chemical_compound
Radiation, Ionizing
cytokine
medicine
Animals
oxidative stress
Cell damage
chemistry.chemical_classification
Reactive oxygen species
biology
apoptosis
General Medicine
medicine.disease
Rats
Oxidative Stress
Radiation Injuries, Experimental
chemistry
Myeloperoxidase
Immunology
biology.protein
Quercetin
Radiation Induced DNA Damage
ionizing radiation
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 10292470 and 10715762
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Free Radical Research
- Accession number :
- edsair.doi.dedup.....09bbae4b119acbc8b4846af7fd49854f