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Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device
- Source :
- Stem Cell Research & Therapy
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Background Mesenchymal stromal cells (MSCs) are multipotent and have great potential in cell therapy. Previously we reported the differentiation potential of human MSCs into hepatocytes in vitro and that these cells can rescue fulminant hepatic failure. However, the conventional static culture method neither maintains growth factors at an optimal level constantly nor removes cellular waste efficiently. In addition, not only is the duration of differentiating hepatocyte lineage cells from MSCs required to improve, but also the need for a large number of hepatocytes for cell therapy has not to date been addressed fully. The purpose of this study is to design and develop an innovative microfluidic device to overcome these shortcomings. Methods We designed and fabricated a microfluidic device and a culture system for hepatic differentiation of MSCs using our protocol reported previously. The microfluidic device contains a large culture chamber with a stable uniform flow to allow homogeneous distribution and expansion as well as efficient induction of hepatic differentiation for MSCs. Results The device enables real-time observation under light microscopy and exhibits a better differentiation efficiency for MSCs compared with conventional static culture. MSCs grown in the microfluidic device showed a higher level of hepatocyte marker gene expression under hepatic induction. Functional analysis of hepatic differentiation demonstrated significantly higher urea production in the microfluidic device after 21 days of hepatic differentiation. Conclusions The microfluidic device allows the generation of a large number of MSCs and induces hepatic differentiation of MSCs efficiently. The device can be adapted for scale-up production of hepatic cells from MSCs for cellular therapy.
- Subjects :
- 0301 basic medicine
Male
Cellular differentiation
Microfluidics
Mesenchymal stromal cells
Cell Culture Techniques
Medicine (miscellaneous)
Clinical uses of mesenchymal stem cells
Gene Expression
Biology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cell therapy
03 medical and health sciences
medicine
Animals
Hepatocyte
Cell Lineage
Cells, Cultured
Microfluidic device
Research
Mesenchymal stem cell
Hepatic differentiation
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Coculture Techniques
Cell biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Liver
Cell culture
Immunology
Hepatic stellate cell
Hepatocytes
Molecular Medicine
Stem cell
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 17576512
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....09b8889261f18ef375ad25732d6dd714