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Trichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic
- Source :
- Experimental Parasitology. 103:44-50
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- Some isolates of Trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-RNA virus. Immune rabbit anti-capsid serum (IRS) reacted with the capsid protein of purified dsRNA virus of a subset of the virus-infected T. vaginalis isolates. A monoclonal antibody (mAb) that recognized the capsid protein reactive with the IRS was generated. Analysis of the virus capsid protein of virus-infected isolates by probing nitrocellulose blots with mAb revealed diversity among immunoreactivity and in the size of the reactive capsid protein. Despite difficulties in visualizing virus within parasites by cross-section electron microscopy, gold-conjugated mAb readily labeled the cytoplasm of virus-positive trichomonads. Finally and importantly, isolates infecting patients attending an STD clinic, 75% of which were virus-positive isolates, had capsid protein of the same size detected by mAb present in all dsRNA viruses.
- Subjects :
- Sexually transmitted disease
medicine.drug_class
viruses
Immunoelectron microscopy
Immunoblotting
Immunology
Sexually Transmitted Diseases
Trichomonas Infections
Monoclonal antibody
medicine.disease_cause
Virus
Mice
Trichomonas vaginalis
medicine
Animals
Humans
RNA Viruses
RNA, Double-Stranded
Mice, Inbred BALB C
Hybridomas
biology
Immune Sera
Antibodies, Monoclonal
RNA virus
General Medicine
biology.organism_classification
Immunohistochemistry
Virology
Infectious Diseases
Capsid
RNA, Viral
Capsid Proteins
Electrophoresis, Polyacrylamide Gel
Parasitology
Viral disease
Subjects
Details
- ISSN :
- 00144894
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Experimental Parasitology
- Accession number :
- edsair.doi.dedup.....099d6ab53c81e2f367763266ec67ceec