Back to Search
Start Over
Development of tetraploidy in V79 spheroids
- Source :
- In vitro. 18(8)
- Publication Year :
- 1982
-
Abstract
- Chinese hamster V-79-171 cells, when placed in suspension culture, spontaneously form multicell spheroids. As the spheroids enlarge the fraction of polyploid (predominantly tetraploid) cells increases and can approach 100% in very large spheroids. Spheroid size, rather than age, seems to be a major determinant for increased ploidy. When cell separation techniques were used to select enriched populations of diploid and tetraploid cells, the growth rate and plating efficiency of the diploid cells was always marginally higher, and they gradually became predominant in mixed monolayer cultures. Cloned tetraploid cells, however, generally remained quite stable, and no consistent ploidy dependent changes in radiosensitivity were observed relative to normal, diploid cell lines.
- Subjects :
- Genetics
Plating efficiency
biology
fungi
Spheroid
food and beverages
Plant Science
DNA, Neoplasm
Neoplasms, Experimental
biology.organism_classification
Models, Biological
Chinese hamster
Cell biology
Cell Line
Polyploidy
Cricetulus
Polyploid
Cell culture
Cricetinae
embryonic structures
Animals
Radiosensitivity
Ploidy
Developmental biology
Biotechnology
Subjects
Details
- ISSN :
- 00735655
- Volume :
- 18
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- In vitro
- Accession number :
- edsair.doi.dedup.....099cd1bf75a950c055e0173261092326