Back to Search
Start Over
Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development
- Source :
- Plant Physiology and Biochemistry, Plant Physiology and Biochemistry, Elsevier, 2009, 47 (4), pp.282-290. ⟨10.1016/j.plaphy.2008.12.004⟩
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- International audience; Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (−)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively. Expression studies had been published but no data were available on enzyme activity. In our work, we devised assays to measure LAR and ANR activity and determine their development throughout the growth of flowers, young berries, and skins of Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them: VvMYB5a and VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of VvMYB5a and VvMYBPA1 was confirmed and specific expression patterns were also established for VvLAR transcripts.
- Subjects :
- 0106 biological sciences
CABERNET-SAUVIGNON
Physiology
Plant Science
Berry
Biology
Reductase
01 natural sciences
Anthocyanidin reductase
Anthocyanins
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Botany
Genetics
FLOWERS
NADH, NADPH Oxidoreductases
Vitis
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
ANTHOCYANIDINE RÉDUCTASE
030304 developmental biology
Plant Proteins
0303 health sciences
PROANTHOCYANIDIN
LEUCOANTHOCYANIDINE RÉDUCTASE
Structural gene
VITIS VINIFERA L
LEUCOANTHOCYANIDIN REDUCTASE
Leucoanthocyanidin reductase
food and beverages
Catechin
Biochemistry
chemistry
Proanthocyanidin
Polyphenol
Fruit
ANTHOCYANIDIN REDUCTASE
SKIN
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 09819428
- Database :
- OpenAIRE
- Journal :
- Plant Physiology and Biochemistry, Plant Physiology and Biochemistry, Elsevier, 2009, 47 (4), pp.282-290. ⟨10.1016/j.plaphy.2008.12.004⟩
- Accession number :
- edsair.doi.dedup.....099b740745da48cbaf998c97a3e603ed