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Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 2, p e0243727 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- In vitro embryo production systems are limited by their inability to consistently produce embryos with the competency to develop to the blastocyst stage, survive cryopreservation, and establish a pregnancy. Previous work identified a combination of three cytokines [fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1)], called FLI, that we hypothesize improve preimplantation development of bovine embryos in vitro. To test this hypothesis, FLI was supplemented into oocyte maturation or embryo culture medium. Embryos were produced in vitro using abattoir-derived oocytes and fertilized with sperm from a single bull known to have high fertility. After an 18–20 h fertilization period, putative zygotes were cultured in synthetic oviductal fluid (SOF) for 8 days. The addition of FLI to the oocyte maturation medium increased (P< 0.05) the dissociation of transzonal projections at 12, 18, and 24 h of maturation, as well as, the proportion of oocytes that reached the metaphase II stage of meiosis. Additionally, lipid content was decreased (P< 0.05) in the blastocyst stage embryo. The addition of FLI during the culture period increased development to the blastocyst stage, cytoskeleton integrity, and survival following slow freezing, as well as, decreased post thaw cell apoptosis (P< 0.05). In conclusion, the supplementation of these cytokines in vitro has the potential to alleviate some of the challenges associated with the cryo-survival of in vitro produced bovine embryos through improving embryo development and embryo quality.
- Subjects :
- Embryology
Physiology
Leukemia Inhibitory Factor
Biochemistry
Zygotes
Cryopreservation
Pregnancy
Animal Cells
Immune Physiology
Medicine and Health Sciences
Insulin-Like Growth Factor I
Cytoskeleton
Innate Immune System
Multidisciplinary
Embryo
Lipids
medicine.anatomical_structure
OVA
embryonic structures
Medicine
Cytokines
Female
Fibroblast Growth Factor 2
Cellular Types
Cellular Structures and Organelles
Embryo quality
Research Article
animal structures
Science
Specimen Preservation
Immunology
Fertilization in Vitro
Biology
Research and Analysis Methods
Andrology
Cryobiology
medicine
Animals
Blastocyst
Embryos
Embryogenesis
Biology and Life Sciences
Embryo culture
Cell Biology
Molecular Development
Embryo, Mammalian
Oocyte
In Vitro Oocyte Maturation Techniques
Germ Cells
Specimen Preparation and Treatment
Immune System
Oocytes
Cattle
Blastocysts
Leukemia inhibitory factor
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....0992b73f51b37c33ade4ed5408fb1332