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The bacteriology in adult patients with pneumonia and parapneumonic effusions: increased yield with DNA sequencing method
- Source :
- European Journal of Clinical Microbiology & Infectious Diseases. 38:297-304
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The aim of this study was to use a 16S rDNA sequencing method in combination with conventional culture in patients with parapneumonic effusions (PPE) to evaluate the methods, study the microbiological spectrum, and examine the presence of bacteria within the different stages of PPE. Adults with community-acquired pneumonia (CAP) and PPE (n = 197) admitted to the Departments of Infectious Diseases at four hospitals in Stockholm County during 2011–2014 were prospectively studied. All patients underwent thoracentesis. Twenty-seven non-infectious pleural effusions were used as controls. The pleural samples were analyzed with culture, 16S rDNA sequencing, pH, glucose, and lactate dehydrogenase. Microbiological etiology was found in 99/197 (50%) of the patients with mixed infections in 20 cases. The most common pathogens were viridans streptococci (n = 37) and anaerobic bacteria (n = 40). Among the 152 patients with both methods performed, 26/152 (17%) and 94/152 (62%) had bacteria identified with culture and 16S rDNA sequencing respectively (p
- Subjects :
- Adult
Male
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Adolescent
medicine.medical_treatment
030106 microbiology
Thoracentesis
Sensitivity and Specificity
Parapneumonic effusion
Bacteria, Anaerobic
Young Adult
03 medical and health sciences
0302 clinical medicine
Medical microbiology
RNA, Ribosomal, 16S
Internal medicine
Pneumonia, Bacterial
Bacteriology
Humans
Medicine
030212 general & internal medicine
Aged
Aged, 80 and over
Sweden
Bacteriological Techniques
Bacteria
biology
business.industry
Sequence Analysis, DNA
General Medicine
Middle Aged
Viridans Streptococci
medicine.disease
biology.organism_classification
Community-Acquired Infections
Pleural Effusion
Pneumonia
Infectious Diseases
Molecular Diagnostic Techniques
Viridans streptococci
Female
Anaerobic bacteria
business
Subjects
Details
- ISSN :
- 14354373 and 09349723
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Accession number :
- edsair.doi.dedup.....098af9803d9b1ad874ba4676a9086e39