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Xylanase modulates the microbiota of ileal mucosa and digesta of pigs fed corn-based arabinoxylans likely through both a stimbiotic and prebiotic mechanism
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 1, p e0246144 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- The experimental objective was to characterize the impact of insoluble corn-based fiber, xylanase, and an arabinoxylan-oligosaccharide on ileal digesta and mucosa microbiome of pigs. Three replicates of 20 gilts were blocked by initial body weight, individually-housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF), a 30% corn bran high-fiber control (HF), HF+100 mg/kg xylanase (HF+XY), and HF+50 mg/kg arabinoxylan oligosaccharide (HF+AX). Gilts were fed their respective treatments for 46 days. On day 46, pigs were euthanized and ileal digesta and mucosa were collected. The V4 region of the 16S rRNA was amplified and sequenced, generating a total of 2,413,572 and 1,739,013 high-quality sequences from the digesta and mucosa, respectively. Sequences were classified into 1,538 mucosa and 2,495 digesta operational taxonomic units (OTU). Hidden-state predictions of 25 enzymes were made using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUST2). Compared to LF, HF increased Erysipelotrichaceae_UCG-002, and Turicibacter in the digesta, Lachnospiraceae_unclassified in the mucosa, and decreased Actinobacillus in both (QQFaecalibacterium by nearly 6 log2-fold change, compared to HF. Relative to HF, HF+XY increased genera Bifidobacterium, and Lactobacillus, and decreased Streptococcus and Turicibacter in digesta (QBifidobacterium and decreased Escherichia-Shigella in the mucosa (QQ
- Subjects :
- 0301 basic medicine
Swine
medicine.medical_treatment
Enzyme Metabolism
Biochemistry
chemistry.chemical_compound
Lactobacillus
Arabinoxylan
Medicine and Health Sciences
Food science
Intestinal Mucosa
Enzyme Chemistry
Bifidobacterium
Mammals
chemistry.chemical_classification
Xylose
Multidisciplinary
biology
Organic Compounds
Monosaccharides
Eukaryota
Genomics
04 agricultural and veterinary sciences
Chemistry
Medical Microbiology
Vertebrates
Physical Sciences
Xylanase
Medicine
Xylans
Research Article
Science
Carbohydrates
Microbial Genomics
Microbiology
03 medical and health sciences
Bacterial Proteins
Ileum
Genetics
medicine
Animals
Gene Prediction
Nutrition
Endo-1,4-beta Xylanases
Bacteria
Bran
Prebiotic
Gut Bacteria
Organic Chemistry
Lachnospiraceae
Organisms
Chemical Compounds
0402 animal and dairy science
Biology and Life Sciences
Computational Biology
Fatty acid
Genome Analysis
biology.organism_classification
Animal Feed
040201 dairy & animal science
Diet
Gastrointestinal Microbiome
030104 developmental biology
chemistry
Amniotes
Enzymology
Microbiome
Zoology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....096c15d034986cea439f64cbf36766de