L-isoform but not S-isoform of myelin associated glycoprotein promotes neurite outgrowth of mouse cerebellar neurons

ELSEVIER, Shimizu-Okabe, C; Matsuda, Y; Koito, H; Yoshida, S, NEUROSCIENCE LETTERS, 331(3), 203-205, 2001. author
Myelin associated glycoprotein (MAG) has growth promoting effect on mouse cerebellar neurons. In the present study, we examined which isoform of MAG has the effect. cDNA for L-MAG and S-MAG was stably transfected into BALB/c 3T3 cells, on which cerebellar neurons were cultured. The neurons were stained with antibody against microtubule-associated protein-2 (MAP-2). Neurites of the neurons cultured on cells expressing L-MAG extended significantly further than those cultured on cells expressing S-MAG or on control cells. Therefore, intracellular domain of MAG may have the potential to affect MAG-neurite interaction.