Back to Search
Start Over
Phosphatidylinositol 4-phosphate 5-kinase β controls recruitment of lipid rafts into the immunological synapse
- Publication Year :
- 2016
- Publisher :
- American Association of Immunologists, 2016.
-
Abstract
- Phosphatidylinositol 4,5-biphosphate (PIP2) is critical for T lymphocyte activation serving as a substrate for the generation of second messengers and the remodeling of actin cytoskeleton necessary for the clustering of lipid rafts, TCR, and costimulatory receptors toward the T:APC interface. Spatiotemporal analysis of PIP2 synthesis in T lymphocytes suggested that distinct isoforms of the main PIP2-generating enzyme, phosphatidylinositol 4-phosphate 5-kinase (PIP5K), play a differential role on the basis of their distinct localization. In this study, we analyze the contribution of PIP5Kβ to T cell activation and show that CD28 induces the recruitment of PIP5Kβ to the immunological synapse, where it regulates filamin A and lipid raft accumulation, as well as T cell activation, in a nonredundant manner. Finally, we found that Vav1 and the C-terminal 83 aa of PIP5Kβ are pivotal for the PIP5Kβ regulatory functions in response to CD28 stimulation.
- Subjects :
- 0301 basic medicine
Immunological Synapses
Phosphatidylinositol 4-phosphate
T-Lymphocytes
T cell
Immunology
Biology
Lymphocyte Activation
Real-Time Polymerase Chain Reaction
Transfection
Immunological synapse
03 medical and health sciences
chemistry.chemical_compound
Membrane Microdomains
0302 clinical medicine
CD28 Antigens
Image Processing, Computer-Assisted
medicine
Humans
Immunology and Allergy
Phosphatidylinositol
Proto-Oncogene Proteins c-vav
Lipid raft
Microscopy, Confocal
Kinase
CD28
Actin cytoskeleton
Cell biology
Isoenzymes
Phosphotransferases (Alcohol Group Acceptor)
030104 developmental biology
medicine.anatomical_structure
chemistry
lipids (amino acids, peptides, and proteins)
Antigens, CD28
030215 immunology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....09303f685083ae9cfa30a43af5d06b67