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B cell dissemination patterns during the germinal center reaction revealed by whole-organ imaging
- Source :
- The Journal of Experimental Medicine, Journal of Experimental Medicine
- Publication Year :
- 2019
- Publisher :
- Rockefeller University Press, 2019.
-
Abstract
- Antibody-mediated long-lasting protection from harmful pathogens depends on collaboration of immune cells within immunological niches. Stoler-Barak et al. introduce an approach that enables the visualization of all the germinal center niches and activated B cells within intact lymph nodes.<br />Germinal centers (GCs) are sites wherein B cells proliferate and mutate their immunoglobulins in the dark zone (DZ), followed by affinity-based selection in the light zone (LZ). Here, we mapped the location of single B cells in the context of intact lymph nodes (LNs) throughout the GC response, and examined the role of BCR affinity in dictating their position. Imaging of entire GC structures and proximal single cells by light-sheet fluorescence microscopy revealed that individual B cells that previously expressed AID are located within the LN cortex, in an area close to the GC LZ. Using in situ photoactivation, we demonstrated that B cells migrate from the LZ toward the GC outskirts, while DZ B cells are confined to the GC. B cells expressing very-low-affinity BCRs formed GCs but were unable to efficiently disperse within the follicles. Our findings reveal that BCR affinity regulates B cell positioning during the GC response.
- Subjects :
- 0301 basic medicine
Immunology
Receptors, Antigen, B-Cell
Context (language use)
Mice, Transgenic
Technical Advances and Resources
03 medical and health sciences
0302 clinical medicine
Antigen
Cell Movement
Fluorescence microscope
medicine
Immunology and Allergy
Animals
B cell
Research Articles
Cell Proliferation
Mice, Knockout
B-Lymphocytes
Microscopy, Confocal
biology
Chemistry
Cell growth
breakpoint cluster region
Germinal center
Germinal Center
Molecular biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Microscopy, Fluorescence
biology.protein
Lymph Nodes
Antibody
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 15409538 and 00221007
- Volume :
- 216
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....092f34949518241449c1e68377be3b55