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Platelet‐derived extracellular vesicles convey mitochondrial DAMPs in platelet concentrates and their levels are associated with adverse reactions
- Source :
- Transfusion. 59:2403-2414
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- BACKGROUND Whereas platelet transfusion is a common medical procedure, inflammation still occurs in a fraction of transfused individuals despite the absence of any apparent infectious agents. Platelets can shed membrane vesicles, called extracellular vesicles (EVs), some of which contain mitochondria (mito+EV). With its content of damage-associated molecular pattern (DAMP), the mitochondrion can stimulate the innate immune system. Mitochondrial DNA (mtDNA) is a recognized DAMP detected in the extracellular milieu in numerous inflammatory conditions and in platelet concentrates. We hypothesized that platelet-derived mitochondria encapsulated in EVs may represent a reservoir of mtDNA. STUDY DESIGN AND METHODS Herein, we explored the implication of mito+EVs in the occurrence of mtDNA quantified in platelet concentrate supernatants that induced or did not induce transfusion adverse reactions. RESULTS We observed that EVs were abundant in platelet concentrates, and platelet-derived mito+EVs were more abundant in platelet concentrates that induced adverse reactions. A significant correlation (rs = 0.73; p
- Subjects :
- Blood Platelets
Damp
Mitochondrial DNA
Immunology
Inflammation
Platelet Transfusion
030204 cardiovascular system & hematology
Mitochondrion
DNA, Mitochondrial
Extracellular Vesicles
03 medical and health sciences
0302 clinical medicine
Extracellular
medicine
Humans
Immunology and Allergy
Platelet
Innate immune system
Chemistry
Transfusion Reaction
Hematology
Cell biology
Platelet transfusion
medicine.symptom
030215 immunology
Subjects
Details
- ISSN :
- 15372995 and 00411132
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Transfusion
- Accession number :
- edsair.doi.dedup.....0925648d08a4642244e5e26f181010ab