Back to Search
Start Over
Xeno-free proliferation of human bone marrow mesenchymal stem cells
- Source :
- Cytotechnology. 64:301-308
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- The proliferation of human bone marrow mesenchymal stem cells (MSCs) employing xeno-free materials not containing fetal calf serum (FCS) and porcine trypsin was investigated for the regenerative medicine of cartilage using MSCs. Four sequential subcultivations of MSCs using a medium containing 10% FCS and recombinant trypsin (TrypLESelect™) resulted in cell growth comparable to that with porcine trypsin. There was no apparent difference in the cell growth and morphology between two kinds of MSC stored in liquid nitrogen using 10% FCS plus DMSO or serum-free TC protector™. MSCs were isolated from human bone marrow cells, stored in liquid nitrogen, and sequentially subcultivated four times employing conventional materials that included FCS, porcine trypsin, and DMSO, or xeno-free materials that included serum-free medium (MesenCult-XF™), TC protector™ and TrypLESelect™. Cells in the culture using the xeno-free materials maintained typical fibroblast-like morphology and grew more rapidly than the cells in the culture using the conventional materials, while the cell surface markers of MSCs (CD90 and CD166) were well maintained in both cultures. Chondrogenic pellet cultures were carried out using these subcultivated cells and a medium containing TGFβ3 and IGF1. The pellet culture using cells grown with the xeno-free materials showed an apparently higher gene expression of aggrecan, a chondrocyte marker, than the pellet culture using cells grown with the conventional materials. Consequently, MSCs that are isolated, stored, and grown using the xeno-free materials including the serum-free medium (MesenCult-XF™), TC protector™, and recombinant trypsin (TrypLESelect™) might be applicable for regenerative medicine of cartilage.
- Subjects :
- JAACT Special Issue
Cartilage
Clinical Biochemistry
Mesenchymal stem cell
Biomedical Engineering
Bioengineering
Cell Biology
Biology
Trypsin
Chondrogenesis
Molecular biology
Regenerative medicine
Chondrocyte
medicine.anatomical_structure
Immunology
medicine
CD90
Aggrecan
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 15730778 and 09209069
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Cytotechnology
- Accession number :
- edsair.doi.dedup.....0905d4a034f4633f0e4bb67d86391117