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Compartment-Specific Remodeling Of Splenic Micro-Architecture During Experimental Visceral Leishmaniasis
Compartment-Specific Remodeling Of Splenic Micro-Architecture During Experimental Visceral Leishmaniasis
- Source :
- The American Journal of Pathology
- Publication Year :
- 2011
- Publisher :
- Aperta, 2011.
-
Abstract
- Progressive splenomegaly is a hallmark of visceral leishmaniasis in humans, canids, and rodents. In experimental murine visceral leishmaniasis, splenomegaly is accompanied by pronounced changes in microarchitecture, including expansion of the red pulp vascular system, neovascularization of the white pulp, and remodeling of the stromal cell populations that define the B-cell and T-cell compartments. Here, we show that Ly6C/G(+) (Gr-1(+)) cells, including neutrophils and inflammatory monocytes, accumulate in the splenic red pulp during infection. Cell depletion using monoclonal antibody against either Ly6C/G(+) (Gr-1; RB6) or Ly6G(+) (1A8) cells increased parasite burden. In contrast, depletion of Ly6C/G(+) cells, but not Ly6G(+) cells, halted the progressive remodeling of Meca-32(+) and CD31(+) red pulp vasculature. Strikingly, neither treatment affected white pulp neovascularization or the remodeling of the fibroblastic reticular cell and follicular dendritic cell networks. These findings demonstrate a previously unrecognized compartment-dependent selectivity to the process of splenic vascular remodeling during experimental murine visceral leishmaniasis, attributable to Ly6C(+) inflammatory monocytes. (Am J Palbol 2011, 179:23-29; 1)01: 10.1016/j.ajpath.2011.03.009)
- Subjects :
- White pulp
Pathology
medicine.medical_specialty
Stromal cell
Neutrophils
Short Communication
Blotting, Western
Spleen
Biology
Monocytes
Pathology and Forensic Medicine
Neovascularization
Immunoenzyme Techniques
03 medical and health sciences
Mice
0302 clinical medicine
Reticular cell
Cricetinae
medicine
Animals
Antigens, Ly
Cells, Cultured
030304 developmental biology
0303 health sciences
B-Lymphocytes
Follicular dendritic cells
Mesocricetus
Neovascularization, Pathologic
Macrophages
Antibodies, Monoclonal
Dendritic Cells
Fibroblasts
Flow Cytometry
3. Good health
Mice, Inbred C57BL
medicine.anatomical_structure
Splenic Red Pulp
Splenomegaly
Red pulp
Leishmaniasis, Visceral
Female
medicine.symptom
030215 immunology
Leishmania donovani
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....08e34b64352ed0513e3e5e9d33304131