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Synergistic Effects of A Combined Treatment of Glioblastoma U251 Cells with An Anti-miR-10b-5p Molecule and An AntiCancer Agent Based on 1-(3',4',5'-Trimethoxyphenyl)-2-Aryl-1H-Imidazole Scaffold
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 5991
- Publication Year :
- 2022
-
Abstract
- (1) Background: In the development of new and more effective anticancer approaches, combined treatments appear of great interest. Combination therapy could be of importance in the management of glioblastoma (GBM), a lethal malignancy that accounts for 42% of cancer of the central nervous system, with a median survival of 15 months. This study aimed to verify the activity on a glioblastoma cancer cell line of one of the most active compounds of a novel series of tubulin polymerization inhibitors based on the 1-(3′,4′,5′-trimethoxyphenyl)-2-aryl-1H-imidazole scaffold, used in combination with a miRNA inhibitor molecule targeting the oncomiRNA miR-10b-5p. This microRNA was selected in consideration of the role of miR-10b-5p on the onset and progression of glioblastoma. (2) Methods: Apoptosis was analyzed by Annexin-V and Caspase 3/7 assays, efficacy of the anti-miR-10b-5p was assessed by determining the miR-10b-5p content by RT-qPCR. (3) Results: The results obtained show that a “combination therapy” performed by combining the use of an anti-miR-10b-5p and a 1-(3′,4′,5′-trimethoxyphenyl)-2-aryl-1H-imidazole derivative is an encouraging strategy to boost the efficacy of anticancer therapies and at the same time to reduce side effects.
- Subjects :
- 4′
Antineoplastic Agents
5′-trimethoxyphenyl)-2-aryl-1H-imidazole
Catalysis
microRNA
anti-miR
miR-10b-5p
glioma
apoptosis
tubulin
1-(3′,4′,5′-trimethoxyphenyl)-2-aryl-1H-imidazole
combination therapy
Cell Line
Inorganic Chemistry
1-(3′
Antagomirs
Tumor
Cell Proliferation
Gene Expression Regulation
Imidazoles
Glioblastoma
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Organic Chemistry
General Medicine
Computer Science Applications
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 5991
- Accession number :
- edsair.doi.dedup.....08dd8a1d03cfce3417bb7a9f6f335091