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Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family
- Source :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname
- Publication Year :
- 2013
-
Abstract
- We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus necrotic ringspot virus, Cucumber mosaic virus and Cowpea mosaic virus. We have analysed the capacity of the heterologous MPs to systemically transport the corresponding chimeric AMV genome. All MPs were competent in systemic transport but required the fusion at their C terminus of the coat protein-interacting C-terminal 44 aa (A44) of the AMV MP. Except for the TMV MP, the presence of the hybrid virus in upper leaves correlated with the capacity to move locally. These results suggest that all the MPs assigned to the 30K superfamily should be exchangeable not only for local virus movement but also for systemic transport when the A44 fragment is present.<br />We thank L. Corachan for her excellent technical assistance. This work was supported by the Spanish granting agency DGICYT via grant BIO2011-25018 and by the Generalitat Valenciana via grant PROMETEO 2011-003.
- Subjects :
- Gene Expression Regulation, Viral
viruses
Green Fluorescent Proteins
Virus Replication
Virus
Cucumber mosaic virus
Brome mosaic virus
Doença de planta
Gene Expression Regulation, Plant
Virology
Plant virus
Alfalfa mosaic virus
Tobacco
Tobacco mosaic virus
Movement protein
biology
Expressão genética
Cowpea mosaic virus
Vírus
biology.organism_classification
Plants, Genetically Modified
Recombinant Proteins
Alfafa
Plant Viral Movement Proteins
Protein Transport
RNA, Viral
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname
- Accession number :
- edsair.doi.dedup.....08ceddcbabdd2ec5a3ead48e7e8967b3