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Intercellular adhesion molecule-3 is the predominant co-stimulatory ligand for leukocyte function antigen-1 on human blood dendritic cells
Intercellular adhesion molecule-3 is the predominant co-stimulatory ligand for leukocyte function antigen-1 on human blood dendritic cells
- Source :
- European Journal of Immunology. 25:2528-2532
- Publication Year :
- 1995
- Publisher :
- Wiley, 1995.
-
Abstract
- Haematology/Immunology Research Group, Christchurch Hospital and Christchurch School of Medicine, Christchurch, New Zealand ICRF Laboratories, Institute of Molecular Medicine, Oxford, GB Dendritic cells (DC) are potent stimulators of primary T lymphocyte responses to foreign antigen. The initial DC-T lymphocyte interaction involves the binding of the adhesion molecule leukocyte function antigen-1 (LFA-1; CDlldCD18) on the T lymphocyte to an intercellular adhesion molecule (ICAM) on DC. Although blood and tonsil DC express ICAM-1 (CD54) and ICAM-2 (CD102) on their surface, anti-ICAM-1 and anti-ICAM-2 monoclonal antibodies (mAb) have little inhibitory activity on the DC-stimulated mixed leukocyte reaction (MLR). We therefore examined the expression of the more recently identified LFA-1 ligand, ICAM-3 (CD50), in comparison to ICAM-1 and ICAM-2 on blood DC and sought a functional role for ICAM-3 in DC-mediated T lympho- cyte responses. Resting blood DC expressed significantly more ICAM-3 than ICAM-1 or ICAM-2 as assessed by flow cytometry. Treatment of resting DC with interferon-y led to increased expression of ICAM-1; however, ICAM-2 and ICAM-3 levels remained relatively constant. Solid-phase recombinant chimeric molecules ICAM-1-, ICAM-2- and ICAM-3-Fc were able to co-stimulate CD4' T lymphocyte proliferation in conjunction with suboptimal solid-phase CD3 mAb 64.1. However, the anti-ICAM-3 mAb CAL 3.10 inhibited a DC- stimulated MLR to a greater extent than anti-ICAM-1 or anti-ICAM-2 reagents and appeared to act by blocking the DC ICAM-3- T lymphocyte LFA-1 interac- tion. As ICAM-3 is the predominant LFA-1 ligand on resting blood DC, we pos- tulate that DC may utilize ICAM-3 for initial DC- T lymphocyte interactions, and that ICAM-1, which is up-regulated upon DC activation, and/or ICAM-2, may contribute to DC migration or later phases of the T lymphocyte activation process.
- Subjects :
- biology
medicine.diagnostic_test
CD3
Lymphocyte
Immunology
Dendritic Cells
T lymphocyte
Dendritic cell
Intercellular Adhesion Molecule-1
Ligands
Intercellular adhesion molecule
Antigens, Differentiation
Lymphocyte Function-Associated Antigen-1
Flow cytometry
Cell biology
medicine.anatomical_structure
Co-stimulation
Antigen
Antigens, CD
biology.protein
medicine
Humans
Immunology and Allergy
Cell Adhesion Molecules
Cells, Cultured
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....08cec2cac66c38bacf193e2d0fa8877c
- Full Text :
- https://doi.org/10.1002/eji.1830250918