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Radiation-induced chromosomal aberrations in mouse 10T1/2 cells: dependence on the cell-cycle stage at the time of irradiation
- Publication Year :
- 1994
- Publisher :
- Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245, 1994.
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Abstract
- Cell-cycle stage radiosensitivity for the induction of chromosome aberrations has been investigated in C3H 10T1/2 cells. Exponentially growing cells were irradiated with 3 Gy X-rays (80 kVp) or 0.6 Gy alpha-particles (LET = 101 keV/micron). The two doses produce the same survival level (37%) in the asynchronous population. Cells were harvested at four different times following irradiation and cell-cycle phase at the time of irradiation was assessed by using the differential replication staining technique. The frequency of chromosome aberrations produced in a given stage of the cell cycle was not constant as a function of the sampling time, but this could not be simply related to the existence of subphases exhibiting different radiosensitivity, because of cell-cycle perturbation introduced by radiation. X-radiation induced more exchanges than deletions, whereas a predominance of isochromatid deletions was observed after alpha-irradiation. This can be interpreted on the basis of the different patterns of energy deposition of densely- and sparsely-ionizing radiation. Both X- and alpha-rays produced a significant increase in the frequency of Robertsonian translocations when cells were exposed in G1 or S phase, but not in G2 phase.
- Subjects :
- G2 Phase
Radiobiology
Population
Biology
Chromatids
Models, Biological
Radiation Tolerance
Chromosomes
S Phase
Mice
medicine
Mitotic Index
Animals
Radiology, Nuclear Medicine and imaging
Irradiation
Radiosensitivity
education
Fibroblast
Cells, Cultured
Chromosome Aberrations
education.field_of_study
Mice, Inbred C3H
Radiological and Ultrasound Technology
business.industry
Cell Cycle
G1 Phase
Chromosome
Cell cycle
Fibroblasts
Alpha Particles
Molecular biology
medicine.anatomical_structure
Cell culture
Nuclear medicine
business
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....088c32bf87678057df4c95403c0e855b