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Emergence of IMP-8-Producing Comamonas thiooxydans Causing Urinary Tract Infection in China
- Source :
- Frontiers in Microbiology, Vol 12 (2021), Frontiers in Microbiology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- The emergence of carbapenem resistance (CR) caused by hydrolytic enzymes called carbapenemases has become a major concern worldwide. So far, CR genes have been widely detected in various bacteria. However, there is no report of CR gene harboring Comamonas thiooxydans. We first isolated a strain of an IMP-8-producing C. thiooxydans from a patient with urinary tract infection in China. Species identity was determined using MALDI-TOF MS analysis and carbapenemase-encoding genes were detected using PCR. The complete genomic sequence of C. thiooxydans was identified using Illumina Novaseq and Oxford Nanopore PromethION. Antimicrobial susceptibility analysis indicated that the C. thiooxydans strain ZDHYF418 was susceptible to imipenem, intermediate to meropenem, and was resistant to aztreonam, fluoroquinolones, and aminoglycosides. The blaIMP–8 gene was chromosomally located, and was part of a Tn402-like class 1 integron characterized by the following structure: DDE-type integrase/transposase/recombinase-tniB-tniQ-recombinase family protein-aac(6′)-Ib-cr-blaIMP–8-intI1. Phylogenetic analysis demonstrated that the closest relative of ZDHYF418 is C. thiooxydans QYY (accession number: CP053920.1). We detected 330 SNP differences between ZDHYF418 and C. thiooxydans QYY. Strain QYY was isolated from activated sludge in Jilin province, China in 2015. In summary, we isolated a strain of C. thiooxydans that is able to produce IMP-8 and a novel blaOXA. This is the first time that a CR gene has been identified in C. thiooxydans. The occurrence of the strain needs to be closely monitored.
- Subjects :
- Microbiology (medical)
Imipenem
lcsh:QR1-502
Aztreonam
Integron
Microbiology
lcsh:Microbiology
Comamonas thiooxydans
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
medicine
antimicrobial resistance
comparative genomic analysis
Gene
Transposase
Original Research
030304 developmental biology
Genetics
0303 health sciences
bla IMP –8
biology
Phylogenetic tree
030306 microbiology
Accession number (library science)
complete genome sequence
chemistry
biology.protein
blaIMP–8
medicine.drug
Subjects
Details
- ISSN :
- 1664302X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....0887d1b2ee7dc3cda05ea49134eb3721